... For large-scale screening, sgRNA libraries are useful for targeting thousands of long ncRNAs at the same...While CRISPR/Cas9 has proven to be an extremely useful system for modifying and assessing ncRNA function...or upregulate ncRNA expression. This approach is useful for lncRNAs because they regulate nearby genes ...effects. Newer Cas protein variants may also be useful for targeting ncRNAs. For example, the Cas12a variant...

...popular, but how do I know if a plasmid is actually useful to me? Plasmid purpose: This is a brief description...easy for scientists to see if the plasmid would be useful for their own research, and helps requestors use...cloning. Cloning information: This section provides useful information about how the vector was assembled,...info you provide in the deposit process, the more useful your plasmid pages will be to your colleagues. ...

...for each group and topic. For example, it may be useful to adapt the second version (multiple presenter...perhaps even two articles per meeting) would be useful. On the other hand if the journal club occurs with...solicit feedback. To ensure that the journal club is useful for the widest audience, you should get feedback... with any recurring meeting, a journal club’s usefulness should be continually evaluated and assessed ...

...the field is currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system to resensitize...can be used to target the sensitive population. Yosef et al.’s proof-of-concept work targeted two distinct...sensitive bacteria from acquiring resistance genes. Yosef et al. suggest that it could be valuable in medical... 25417800. PubMed Central: PMCID PMC4282009. 4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron. “...

...Curate and present some useful data Typically lists and pictures are the most useful parts of reviews. These... was found and the size of the study). This was useful because you could easily see how frequently each...information was available in GenBank, but it was useful to present it all in one place to get a sense of...

...Mortensen et al., 2019). Our method might provide some useful guidelines for the development of transient transformation... If you are new to the field, the following are useful tips for your first transient leaf transformation...β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves...reporters to measure promoter activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90...

... stability in vivo. However, Spinach has proven useful in the engineering of riboswitches. Riboswitches...thermal stability, making it a potentially more useful tool for in vivo studies. Dimerization of Broccoli...more photostable than Spinach and Broccoli, and useful for quantitative studies. Specifically, Corn was... compared to Mango, and all are photostable and useful for both live and fixed cell imaging. New ingredients...

...and new databases are supporting their access to useful resources during this time. Facilitate collaborations...fight COVID-19. They’ve also collated a list of useful research resources.  "We realized that there is... lists data and experimental resources that are useful to any researcher studying COVID-19. “Open scientific...

...calcium sensors from Janelia (jGCaMP7) continue to be useful for monitoring neuronal activity in the form of...while maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations...AAV1) Optogenetics Optogenetics continues to be a useful method for manipulating specific neurons. We recently...

...being developed and we are excited to offer these useful tools to the community. Among other new biosensor...Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human primates, where fusion...to our recombinase AAV inventory. Flp is another useful recombinase that can be used in combination with...

... directly what protocols would be useful. Think GCE could be useful in your research? We definitely recommend... scientists on which protocols would be broadly useful for biomedical researchers. One way of doing that...

...continue to provide and expand this diverse and useful collection of tools so that researchers around ...Curious which viruses researchers have found the most useful so far? We crunched the numbers on our Viral Service... made in your own lab. We provide protocols and useful tips for generating your own lentivirus. Secondly...

...important neuronal signalling pathway - if you’ve got a useful tool, there’s likely someway you can apply it in...lentivirus are now available from Addgene. While super useful, these vectors require a bit of expertise and can...learn more about these tools and to tell us how useful the service has been for their experiments. This...

... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...Comments on what a given line of code does are very useful for those that are just starting out, and helps...line history by recording all commands entered is useful for beginners who are still learning the basics...

...Monster RANbody doesn’t recognize the scaffold and is useful for detecting GFP. Spaghetti Monster tagged RANbodies...IgY domains. Chicken IgY domains are especially useful for multi-color staining of tissue because they...RANbodies. If you know of a nanobody that would make a useful RANbody, consider leaving a link to its sequence...

...characterized parts into a TU! What is modular cloning useful for? All of this sounds really neat — but when ...when is this type of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose... promoters, enhancers, etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many...

...typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they infect...connections. While this transsynaptic spread is useful for neuronal tracing, it has two practical limitations...neurons doesn’t reveal network connections in a useful way, but rather just labels the whole brain. To...

...potentially a photograph of yourself. A photo can be very useful in the event that you will not be physically present...readable from several feet away. It can also be useful to use two different font styles; one for your ...The Thompson Rivers University Library provides useful tips on how to craft this type of poster using ...

...quantitative colocalization analysis methods and useful image analysis software tools. Pitfalls, limitations...colocalization methods Fluorescent proteins are very useful tools, but they are not without limitations and...you find the resources discussed in this article useful for your next localization experiment and invite...

... basics of multicistronic vectors: why they are useful, how they work, and how to get started with them...exclusively be used as a means of detection; they are useful almost anytime you want to express multiple genes...independent of the 5’ cap.  IRES elements are very useful and commonly found in bicistronic vectors; however...