Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 927 results for: TIM;

Showing: 61 - 80 of 927 results

  1. TALEN Guide

    Type
    Collection
    ...acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that determines which single ...

  2. Lentiviral Prep Service

    Type
    Collection
    ...Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...Therefore, codon optimization may disrupt the fine-tuned timing of translation and ultimately protein function...Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz Codon Optimization  ATUM DNA2.0  IDT Synthetic...Nakamura et al., 2000).  What is codon optimization? Codon optimization is an approach in gene engineering...when do you want to codon optimize your gene of interest? Codon optimization has become extremely useful... worth codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression...codon optimized membrane protein expressed in mammalian cells. In this study, codon optimization resulted...and concerns: When not to codon optimize? Although codon optimization increases protein production in ...

  4. CRISPR Plasmids - Plants

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...blocking time! Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting...Temperature and time You may need to optimize your antibody incubation temperature and time. Though the most...talk about western blot optimization and troubleshooting.   Before you can optimize or troubleshoot a western...long transfer time. Be aware that the transfer works by electricity, so long transfer times increase the...blocking times or higher concentrations of blocking buffer. If you see a low signal, you can optimize blocking...backgrounds.  Once you’ve optimized antibody concentration, you can optimize ‌incubation conditions to...important to let them wash for the full amount of time, every time. Imaging The last step in a western blot tends...

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...was repeated two or more times in tandem. Such plasmids are known as multimers, and they are the result... in a process known as plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology...various multimeric forms (e.g. concatemers, dimers, trimers, tetramers). Since then, multimerization has ...plasmid multimers function just as well as monomers. However, due to their larger size, multimers may exhibit... detecting plasmid multimers. Digests themselves cannot be used to detect multimers, since restriction...frequency of multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook...a lower temperature such as 30°C or optimizing the incubation time can help maintain cultures in log phase...

  8. Brain Armamentarium

    Type
    Collection
    ..._3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) Optimized expression of SYFP2 (yellow fluorescent protein...

  9. Lentivirus Plasmids

    Type
    Collection
    ...-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under...

  10. The time and cost required to make a plasmid

    Type
    Blog Post
    ...than not!), we think it’s about time we updated this post. How much time does it take to make a plasmid...traditional “one at a time” approach. According to his math, it takes five hours of hands-on time to make a single...decrease plasmid production time and cost If you’re short on money or time, there are a few options you...order to preserve time and money for their own experiments. Keep good records Oftentimes, you’ll be able...how long it takes to make a plasmid? Or how much time you have to spend cloning before you can start your...What about all the reagents you need to order? Sometimes, it feels like an eternity of cloning, waiting...shared milestone in 2018, we wrote a post on the time and cost required to make a plasmid. In 2023 (and...

  11. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...just end up spending more time in the lab. Obviously I didn’t have the best time management skills, but ... manage my time. As a busy researcher, you’ve probably already started coming up with time management ...planning, and procrastination. Time management through prioritiziation Every time you begin a task, you’re ...specific amounts of time to specific tasks beforehand and assess whether that time was sufficient for ...then you should definitely allot more time for that task the next time around. Finally, when prioritizing...some help. Today in our transferable skills guide: Time Mangement for Scientists. Back when I used to do...experiments in graduate school, I had to divide my time between lab work and practicing my SciComm skills...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...your plasmid yield is sub-optimal? If you are consistently getting sub-optimal plasmid yields from your...it will likely save time in the long run! While the choice of strain will ultimately depend on the particular...incubation time can improve the cell density and therefore the plasmid yield. You can optimize your growth...more to achieve maximum plasmid yield. The optimal growth time should be determined for each plasmid/strain... post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your stab...your prep, try our tips below! How to optimize your plasmid prep We'll discuss the following techniques ...strain to see if additional steps are needed to optimize yield. You might want to include an extra wash...

  13. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTTCCGATGGATGAAGTTattgacgatcttattgGCCTTGAAAACGGGTTTA TIMM22 TAL3578 & TAL3579 TTGTTCTGGGAGGTGCTtttggagtcttcacagCCGGCATTGATGCAAA...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...localization changes over time.Hallmarks of fluorescent protein timers When FP timers are first expressed,...expression time. 2. Timer functionality with a single gene Although it is possible to make a timer system ...FPs, the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers...population of timer indicates the age of the pool: the higher the ratio, the older the pool. FP timers have been... hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of mature to ...immature fluorescence from the FP timer is dependent on the total expression time but independent of protein ...total expression time can therefore be used to determine how long a pool of FP timers has been expressed...

  15. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...hopefully help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque...supporting their access to useful resources during this time. Facilitate collaborations within the scientific...highly skilled scientists, willing to volunteer their time and expertise for this cause: from virologists who...

  16. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... Working with plants doesn’t always have to be a time-consuming process. While developing transgenic hairy...transformation process could save the plant biologist some time… months, in fact. In the Lee-Parsons lab, we are...plant, the Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production...C. roseus by working with young seedlings and optimizing several other aspects of the transient transformation...available for your species This will save you a lot of time. Don't re-invent the wheel. Test different leaf ...

  17. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... slowly after introducing a cut, with residence times on DNA of approximately 6 hours. To our surprise...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery." Elife 3 (2015): e04766...

  18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...determine when the optimal time to conduct an experiment begins. This can save you time in the long run ...information.  Optimize titer for the DIO/FLEx vector Once you have determined the optimal injection parameters...begin to optimize the DIO/FLEx vector. This step is essentially the same as what was used to optimize the ...used.   Following this stepwise optimization will take a fair amount of time, and it may be tempting to skip...experiments. However, by optimizing all conditions first, you will save time, frustration, and funds in...the causes of off-target expression and ways to optimize experiments to minimize it. Overview of DIO/FLEx...created with BioRender.com   Strategies for optimizing expression So now that we know spontaneous recombination...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...which takes time because the solution is viscous, or by autoclaving, which also takes time. Rather than... virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great...total, this process takes 4-7 days, not counting the time needed to expand the HEK293 cells. Check out the...screening for ITR deletion is still necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer... my AAV packaging PEI transfections. It saves me time and helps me figure out if I have enough plasmid...the HEK293 cell culture supernatant because it’s time consuming to prepare the 40% PEG solution. PEG takes...AAV lysis buffer and freeze/thaw the lystate four times in a 95% ethanol and dry ice bath to release the...

  20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...selected sgRNAs in this optimal window and ranked them based on their optimized sgRNA rules and potential...genetic screen. However, there are benefits to optimized libraries that utilize only a few sgRNAs per gene...Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the rules identified...library performance. The Root and Doench lab’s optimized genome-wide CRISPRko, CRISPRi, and CRISPRa libraries... a look at the features and advantages of the optimized human CRISPR libraries. Brunello: human CRISPRko... Thus, the Brunello library proves that a few optimized sgRNAs is enough for efficient library performance...Overall the Root and Doench lab’s have proven their optimized, human genome wide CRISPRko, CRISPRi, and CRISPRa...
Showing: 61 - 80 of 927 results