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We narrowed to 198 results for: aav vector plasmid

Showing: 61 - 80 of 198 results

  1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable... in a single lentiviral transfer vector or separate transfer vectors. May contain reporter gene (e.g. ...non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...Components of System Application Mammalian expression vector Promoter driving Cas9 expression can be constitutive...generate stable cell lines. Packaging and Envelope plasmids provide the necessary components to make lentiviral...for conducting genome-wide screens using CRISPR. AAV transduction Only compatible with SaCas9 (packaging...

  2. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. ... Protocols AAV Titration by qPCR Using SYBR Green Technology AAV Titration by qPCR Using SYBR Green Technology...also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction The AAV Titration protocol... calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation Size...Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...

  3. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...separate plasmids and expressed via cells cultured with the vectors. This allows the viral vectors to reproduce...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral vectors are,...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...101: Viral Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's...

  4. Reflections and Looking Ahead

    Type
    Blog Post
    ...response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a service... and simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment... our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing...next-generation sequencing, setting new standards for plasmid quality control. Through our partnership with the...toward giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing...

  5. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery...protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in your...CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types ...Scientists eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career... two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition has now been released...top of the many developments in science and the plasmids, technologies, and researchers behind them. As...most popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to ...

  6. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...page! AAV purification by iodixanol gradient ultracentrifugation This video goes over AAV vector purification...  Check out the AAV Purification protocol page for more detailed instructions. AAV titration by qPCR... given AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock used...service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world...strategy to confirm the identity and purity of all AAV viral lots. Aligning with Addgene’s mission to “... used to generate a standard curve, visit the AAV Titration by qPCR written protocol page. Getting ...

  7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols...Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction ...polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the ... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis... the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single AAV vector...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Gether lab, now available as ready-to-use AAV vectors.   ...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ...

  9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...don't exactly "eat" plasmids as depicted in this comic, when a bacterium uptakes a plasmid, the genes on the... the plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent...phagemids, a DNA cloning vector that contains both bacteriophage and plasmid properties. The phagemids...lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to create both transient cell...can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations at... information on viral vectors, including transduction download our Viral Vectors 101 eBook. Bacterial ... Read all of our Plasmids 101 blog posts Check out all of our blog posts on plasmid cloning Resources...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...modular vector assembly process designed for a quick, plug-and-play approach to cloning CRISPR vectors (McGee...for each module, generate the vector file, and quickly clone their vector of interest. And it truly is ...destination vectors for various delivery methods, along with compatible inserts. All destination vectors contain...heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right for your...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because...least one plasmid was the correct sequence for every individual assembly. What destination vectors are available...

  11. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    ...edition of our Viral Vectors 101 eBook!  This practical guide to using viral vectors in the lab is a helpful...introduction to viral vectors and their elements; resources on different types of vectors, capsids, and tools...everything from producing your own vectors in the lab to optimizing AAVs in your experiment.      Our second...en español Plasmids 101 eBook 4th edition Resources on addgene.org  Addgene's Viral Vectors Guide Addgene's...

  12. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...injection site from the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to... Using AAV Technologies Using AAV for neuronal tracing Resources on Addgene.org Read our AAV guide Browse... Browse all  AAV vectors Find ready-to-use AAV preps ...growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we invite you...contributed to the AAV Data Hub. If you haven’t yet, we encourage you to share your AAV data on the hub,... on AAV data hub! Related resources on the Addgene blog Important Considerations When Using AAVs Three... celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as an open...

  13. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions...Baradaran’s expertise in viral safety, gene therapy vector development, vaccine development, and assay technologies...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed...Melina Fan and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy....

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...fluorescence from other regions of the cell. By combining vectors expressing different fluorescent proteins targeting...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    ...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing... 2025, we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...nonprofit that would facilitate the sharing of plasmids between scientists. The service would allow people...Addgene before we were a “labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP... our first major repository milestone — 10,000 plasmids deposited and available — and nearly seven before...possible for scientists to both deposit and request plasmids, was a major focus of our first decade. We did...

  16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base editors...editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their... Figure 1: The base editor is split into two plasmids and co-transfected into cells for reconstitution...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...

  17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...calcium.”  Primary Vector Type The vector type should describe how your plasmid is intended to be used...lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids containing the ccdB gene...deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process can be... Send plasmid information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene...spreadsheet and copy and paste your plasmid data directly into our file, one plasmid per row, as outlined by the... Data Field Description Plasmid Name Use a descriptive plasmid name and match the name as it ...application of the plasmid and will help a scientist outside your field understand if this plasmid will work for...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...over 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox 5 and...for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here. ...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting Viral Vectors 101...fact, of how adenoviral vectors work. What are adenoviruses?   Adenoviral vectors are commonly derived from... makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3...usually present in the transfer plasmid for the majority of adenoviral vector systems used in research, they...adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases... only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral vector expression is very...has been reported when using vectors, and immune inactivation of the vector can still occur even in the...

  20. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered... domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter...is also available in several ready-to-use viral vectors.    References and Resources Additional Resources... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...
Showing: 61 - 80 of 198 results