We narrowed to 324 results for: cloning

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

How much time does it take to clone a plasmid and how much does it cost? We do some back of the envelope math to try and figure it out!

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Addgene has reached 100,000 total reagents in the repository! The next milestone coming up is 100,000 and we'd like you to guess the date that happens (a contest).

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Perturb the activity of 17 different signaling pathways using the cancer pathways ORFs kit.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Learn how to produce, purify, and titer adenoviral vectors for your research.

Learn what a high fidelity DNA ligase is and when you might need one.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Interested in sharing your lab protocols with others? Here are 7 software apps that can help you share and edit your protocols with other scientists.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

A round up of guest blog posts of 2024. Thank you to our guest contributors!