We narrowed to 229 results for: cre encoding plasmid

...mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him on twitter...University of Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...the number of fluorescent proteins is steadily increasing. Often, the choice for a fluorescent protein ...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him on twitter...on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles...at the University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools...probes and more. Chapter 5 - mTurquoise II and the creation of improved fluorescent proteins through mutagenesis...

...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him on twitter...University of Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...Cas9-nuclease editing. Typically, a single array encoding the tandemly assembled tRNA-gRNAs is placed downstream...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

...resistance gene being encoded by a high-copy plasmid. In the case of the genomically encoded quinolone resistance...genome is lethal. If this DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which...sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, ...genes in E. coli using plasmid and phagemid delivery systems. Phagemids are plasmids containing a phage origin...sequence results in cell death. Plasmid targeting may result in plasmid loss and antibiotic resensitization...RNAs (crRNAs) targeting either genomic (blue) or plasmid-based sequences (red). Treating a mixed population...specifically targets bacteria with the given genomic or plasmid sequence from a mixed population without affecting...

..."Addgene is one of the leaders in distributing plasmids amongst the academic community. On the BioBricks... flow.' People must be able to use genetically encoded functions - the words in our language for programming... with Endy more about the BIOFAB and BIL gates plasmid kits, the Biobricks Public Agreement, and the future...first to be made available via Addgene and the BPA Plasmid Collection - enable? Endy: The BIOFAB collection...engineering an entire cell. Typically when people use plasmids, they are trying to do one thing at a time. What... tricky to tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic...peptide or RNA aptamers that act as genetically encoded sensors. Those sensors could transduce otherwise...

...once in the plasmid backbone and another enzyme that cuts within the insert. If the plasmid contains the... PCR product if the plasmid contains the insert and no PCR product if the plasmid does not contain the...Addgene blog Get all the plasmid basics in our Plasmids 101 series Find more molecular biology protocols...your desired plasmid. In many cases, you will need a screening strategy as well. In a screen, you aren’t... for example on a plasmid, creates a functional 𝛽-galactosidase. Blue-white screens rely on vectors where...where a  screen comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne...blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase which can hydrolyse...

...transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright...Wright Lab. Collections of plasmids that encode zebrafish receptors or monoclonal antibodies to zebrafish...

...indicators Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype...DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-... constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-...membrane-embedding voltage detector. Find Archon1 and its cre-dependent version at Addgene! Fluorescent protein...be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato...serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5, AAV8  pAAV-hSyn-mCherry...channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA...

...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him on twitter...University of Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach...

...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...10 years of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check...pSV-PGC1 was Addgene's first distributed plasmid and was created in the lab of my thesis advisor, Dr. Bruce...

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...constructs in plasmid form. Fig. 1: Summary of the approach and outcomes of the screen for improved...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and...improved genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The resultant JEDI-2P sensors...viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...

... successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene...including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked by recombination...transformed with the plasmid backbone (with or without your inserted DNA) and further screening will help you...example, Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy...allows the bacteria to grow on sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this... finding more and more ways to creatively perform selections in plasmid cloning. Additional resources...purify your gene of interest, get it into your plasmid backbone, and zap the mixture of DNA into cells...

...Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2...see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV...AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...ll work with you to get the word out about your plasmids and their potential educational uses. We believe...30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional ...developmental biology Use the pathways over time plasmids to engage students in functional genomics research...out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here ...to provide them to their students. My desire to create a set of low-cost, easy-to-use educational biology...fragrances that they can smell, or enzymes that can create hydrogels that they can touch. With BioBits, we...

...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...repair by HDR facilitates in-frame insertion of tag-encoding sequences at the intended target site. With the...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...single-stranded donor DNA (ssODN) that contains the tag-encoding sequence and short homology arms (60-70 bp) is...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...targeting the gene of interest) and ssODN (tag-encoding donor DNA) using our TAG-IN bioinformatics tool.A...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...

...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...glycoprotein. The pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope... spike protein, scientists encode the spike protein on the envelope plasmid. Image from Crawford et al...with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of...

... heavy chain proteins as well as the genes that encode those proteins are designated by the lower-case...co-expressing IgM and IgD isotypes antibodies which are encoded by the heavy-chain constant genes μ and ẟ. These...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector...vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can be...vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, may be...Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral Barcoding...expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium...