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We narrowed to 168 results for: crispr library

Showing: 61 - 80 of 168 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read ...Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas ...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et al., Science...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus...

  2. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop CRISPR editing...their paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation...minimal payloads!) Uncovering Ancestral Bias in CRISPR Research: We spoke to researchers from the Boehm... how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A...

  3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post
    ...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing two fluorescent...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in ...vitro screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune...Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...used for high throughput screens using pooled libraries. Find the CHIME plasmids at Addgene. LaFleur et...

  4. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...be used both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using ...Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783...Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of choice after...

  5. Hot Plasmids - February 2022

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    Blog Post
    ...genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a... Penn State Protein Ladder Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...gene perturbation by: Gabrielle Clouse While many CRISPR-based genetic screening methods have primarily ...10.1038/s41598-021-96051-x Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and...engineering applications. CasMINI, a new miniature CRISPR-Cas system for editing and gene activation, is ...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience, including transgenic...neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant...nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries. However, using... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks...molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for ...

  7. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach." Nature... Out Our CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR Lentivirus...Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of.... To date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating...PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer... Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators: A Practical Guide ... crispr...

  8. CRISPR 101: Off-Target Effects

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    Blog Post
    ...on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook More ...the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The Other...as clonal differences. If you are performing CRISPR library screens, the same principle holds true - the...for unintended consequences in an experiment. For CRISPR work, this often means DNA cleavage at unanticipated...will discuss when off-targets can be an issue in CRISPR, how to predict and minimize them, and ways to ...t be avoided. What are off-target effects? The CRISPR-Cas system is an RNA-guided nuclease that either...sequence similarity to the on-target site. When a CRISPR-Cas system localizes to unintended sites and performs...

  9. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these...etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered)...

  11. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some ...Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website Visit our CRISPR Guide Pages...editors, Casilio, CombiGEM, and a variety of pooled libraries. Not all of these technologies were without controversy...requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name,.... Cas9, of course, is the key component of most CRISPR systems and generates double strange breaks at ...Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281... Pages Browse Our CRISPR Collection Find gRNA Design Tools ...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...be saved to a Semantic Scholar library. You can add hashtags to library entries, which can help you organize...papers Papers are easily saved to a Meta library. The library will suggest additional papers based on ...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...on the papers you have saved. This library can also be synced with a Mendeley reference manager account...

  14. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    Blog Post
    ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale... awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering, Dr. Jennifer Doudna and...Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014 Johnson & Johnson’s Dr. Paul...dinner which took place at the New York Public library. Being in the presence of scientific heroines is...disease. They are finding they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in...technologies. Addgene is proud to be helping get CRISPR reagents out to academic labs. It is our hope that.... He offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus programs...

  15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post
    ...Your gRNA for CRISPR Genome Editing PRIME Editing: Adding Precision and Flexibility to CRISPR Editing Resources...Resources on Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...prime editing efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict...high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled to their target allowing...

  16. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...sequence viewer software and extensive feature library, our updated plasmid and sequence displays are ...features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies a variety...in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...

  17. New and Upcoming Viral Vectors - May 2020

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    Blog Post
    ...available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used...

  18. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...Lab Browse the CRISPR Collection Find CRISPR Pooled Libraries  ...question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but...are a few reasons why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more...targeting flexibility Unlike Cas9 and the other CRISPR enzymes, NgAgo does not require a PAM sequence,...viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded breaks, with either... off-targets More research is needed to compare CRISPR and NgAgo, but it seems that NgAgo has lower potential... crispr...

  19. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources More resources on... DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are powerful tools. CRISPRi uses a... for us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR/Cas9 techniques...Bester 2021.    Alternative CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely...If the CRISPR/Cas9 system is not effective for your experiments, there are plenty of other CRISPR-based...many protein-coding genes thanks to systems like CRISPR/Cas9 that allow them to manipulate full genes and... Knock-out: Targeting short vs. long ncRNAs The CRISPR/Cas9 system is often used to knock-out, or permanently...

  20. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...Addgene: Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to...metabolic biofactories by demonstrating the use of CRISPR-based RNA scaffolds to mimic natural transcriptional...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed...browse/article/6441/ Resources: An open source library of characterized aptamers and supporting publication...
Showing: 61 - 80 of 168 results