We narrowed to 73 results for: gag pol

...or contract, you should be starting to send out polite emails with your CV to people you’d like to work...scientists and technical people) is linked to engagement and access to growth opportunities, not salary...

... from audiences as they do science outreach and engage with the public, and learn from the process of ...broader impacts for their work in education and policy and to draw attention and support to work in their...

...Editing the PAM prevents the prime editor from re-engaging with DNA it has already edited. Created with BioRender.com... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

...engineered SpCas9 variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

...SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, ...159 (3), 647–661. PMID: 25307932 Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford...

... Fig. 2: Prime editor engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand...

..., including investigations into environmental pollutants and health, embryo growth, brain function, and...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

...sharing cells: the black market of cell biology. Engaging in this practice would seem to save time and money...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...When these molecules, called Bispecific T-cell engagers (BiTE®s), bind CD3 on T cells and a tumor-specific...

...lab Writes a lot of grants Varying degrees of engagement with what happens in the lab Official title is...dedicated to different career options from science policy to science communication and much more. I hope ...

... site and contains the desired edit (or edits) engages the prime editor protein. The prime editor consists...transcriptase domain copies the desired edit, directly polymerizing new DNA onto the nicked target strand. The initial...

...vector Forward Polyhedrin forward AAATGATAACCATCTCGC Polyhedrin promoter Forward Polyhedrin reverse GTCCAAGTTTCCCTG...Reverse GAAGAGAAAAACATTAGTTGGC For Pichia vectors with AUG1 promoter Reverse BGH-R TAGAAGGCACAGTCGAGG Bovine...beta-globin intron Reverse Bglob-pA-R TTTTGGCAGAGGGAAAAAGA Rabbit beta-globin polyA region Reverse CAT-R GCAACTGACTGAAATGCCTC...pHybLex Reverse GAGTCACTTTAAAATTTGTATACAC ADH terminator Reverse pLTet-F ACTGAGCACATCAGCAGGAC Lambda phage...-R GAAATTTGTGATGCTATTGC SV40 polyA Reverse SV40pro-F TATTTATGCAGAGGCCGAGG SV40 promoter/origin Forward...TTGTCTCCTTCCGTGTTTCA Thymidine kinase polyA Reverse Tn7-end GGGGTGGAAATGGAGTTTTT Bacteria transposon Tn7 Reverse...Forward V5 Reverse ACCGAGGAGAGGGTTAGGGAT V5 epitope Reverse WPRE-R CATAGCGTAAAAGGAGCAACA 5' end of WPRE Reverse...

...SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, ...159 (3), 647–661. PMID: 25307932 Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford...