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We narrowed to 111 results for: gfp fluorescence reporter

Showing: 61 - 80 of 111 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, ...prep # 55632-AAVrg). pAAV-CAG- FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  2. Lentiviral Prep Service

    Type
    Collection
    ...express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro... 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 .... This library expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag.... This library expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag.... This library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral...on each item's material page. Actual titers are reported with each shipment. For more details about our...

  3. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB...drive expression of either Cre recombinase or a fluorescent marker in the various cell types of the basal...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New serotype pAAV-Ef1a-DIO...

  4. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...apart. While the classic Green Fluorescent Protein (GFP) and other fluorescent proteins revolutionized live-cell...Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien R. Y. (1998). The green fluorescent protein... with a fluorescence-based approach. These challenges can include background autofluorescence from the...yields results that don't reflect true biology. Fluorescent fusions utilizing bulky tags can disrupt normal...the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...This work highlights HiBiT tagging as a sensitive reporter for tracking proteomic cargo flow via EVs. Find...including NanoBRET assays to monitor the binding of fluorescently-labeled VEGF-A to the heteromeric receptor complex...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...the b(right)est fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...stability of fluorescence over time is crucial. For instance, the fluorescence of fluorescein isothiocyanate...principles of fluorescent dyes/ligands Figure 1: Example structures of fluorophores, fluorescent dyes,...dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to the emission...selection and optimization. Fluorescent experiment types: Different types of fluorescent experiments, such as...to the ability to ‘toggle’ between fluorescent and non-fluorescent states in response to a specific stimuli...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    ...Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe)...marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons...colleagues identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide...dimerization while maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023)....Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org..., et al. (2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013... Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31-37...various combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate protein ...library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...’ end of the leader sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers...fusions. Some applications of PUF protein fusions reported in the literature include RNA detection, RNA cleavage...et al 2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase...Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging...Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning and...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to blue ...like chromoproteins, fluorescent proteins, and luminescent proteins. While fluorescent proteins and luminescence...from fluorescent proteins? Before going into the difference between chromoproteins and fluorescent proteins...light, whereas chromoproteins do not fluoresce at all or fluoresce at very low levels. Chromoproteins in...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful... chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way... in nature Compared to the history of fluorescent proteins in molecular biology research, chromoproteins...

  10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ... Posts on Fluorescent Proteins Browse Our Fluorescent Protein Pages Find the mOX Fluorescent Proteins ...protein non-fluorescent. Unfortunately, accumulated misfolded and nonfunctional (dark) fluorescent proteins...Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted via...applicable to many microscopy experiments with fluorescent proteins (FPs) localized to compartments other...that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions on quantitative imaging... and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright signal for... fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent and...

  11. Viral Production

    Type
    Collection
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, ... prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  12. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following...in-frame fluorescent fusion protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

  13. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...neomycin, puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

  14. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...small molecule activates the fluorescence of the small molecule. Fluorescent RNA aptamers are used to track... applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach ...selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution...using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable! ...What is an RNA aptamer? RNA is not inherently fluorescent and can be challenging to track intracellularly... affinity to small molecules to induce their fluorescence. Until a binding event occurs, neither the aptamer... aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its target small...

  15. Serotype Testing AAV

    Type
    Collection
    ...: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of...AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in...plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information... available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing... 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV ...plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...sequence of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in ... the RNA reporter will be cleaved resulting in activation of the fluorophore. The fluorescent signal can... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA ...

  17. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry...30220597. ACE system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing...Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification of base...ratio of mCherry-positive to eGFP positive cells through fluorescence microscopy or flow cytometry. Using...cobalamin-binding riboswitches that bind to cobalamin-fused fluorescent dyes; binding of the cobalamin fusion to the ...the riboswitch results in increased fluorescent signal. Prior to the development of these probes, fluorophore...fluorophore binding aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging...

  18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...such as drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes...epitopes, such as GFP (Paix et al., 2014). A further development of this approach reported in 2017 uses the...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...efficiencies, obtaining as many as 100 independent GFP knock-ins from a single injected animal.  PAM sequence...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...cyan-excitable red fluorescent protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1...as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team ...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating...Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors...to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable monomeric form as determined...

  20. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306 C321...S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid...
Showing: 61 - 80 of 111 results