We narrowed to 68 results for: gfp lenti
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TypeGuide...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...
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Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...from three to two. Lentiviral delivery of BioTAP-tagged dCas9 and gRNAs. Using lentiviral delivery of the...more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs ... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...Multicistronic Element Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A...-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect For the co-expression....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down menu to ...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence...involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence microscopy or... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene...incapable of producing infectious virus (similar to lentiviral vectors) because they do not encode all of the...Addgene.org Browse Our Viral Vector Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide ... -
CRISPR Guide
TypeCollection...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR... -
CRISPR Guide
TypeGuide...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...