We narrowed to 206 results for: kars;

...to as CRISPRa, was initially published in 2013 (Bikard et al., 2013, Perez-Pinera et al., 2013). In the...experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini...

.... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

... system as it relates to its natural role in prokaryotic immunity to viral invasion.  His lab is studying...distinct aspects of the CRISPR regions of the prokaryotic genome. Then he desribed how P. furiosus has ...

...Mustafa, M., Nik Yusof, N. K., Lee, C. K., Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015...

...many different cell types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is ...

...Insect cells can also be used to produce complex eukaryotic proteins with the correct post-translational ...system to generate large quantities of recombinant eukaryotic proteins. Although many species of yeast can ...

...Salvetti A (2005) Evidence for Encapsidation of Prokaryotic Sequences during Recombinant Adeno-Associated... Karen Guerin...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...Mycoplasma Our 293T cell line was obtained directly from Takara, and is routinely tested for mycoplasma contamination...

...However, approximately 40% of human (and most eukaryotic) proteins localize to chemically distinct subcellular...fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway."Biochemical and biophysical...

...interactions. What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent...May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer...

...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

...Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism, is a commonly used model organism in...double every 2 hours. Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable...

...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...

...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest...

...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana... M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein...

...Laboratory. Vancouver, BC Canada 49.2827°N 123.1207°W – Karissa Milbury I attended the march in Vancouver, BC,...science in the public sphere as often as they can. Karissa Milbury is currently a PhD candidate at the BC ...

...for PCR and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but ...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

...luminescence (lux operon) Gram-negative bacteria Attila Karsi 14080 pAKlux2 Promoter activity Luminescence (lux...lux operon) Gram-negative bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a...GFPmut3a) Gram-negative bacteria Attila Karsi 18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP and Venus...Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence ...