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  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  14. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...use the library, the authors packaged it into lentivirus and used the resulting lentiviral library to ...applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing... system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable...
Showing: 61 - 80 of 112 results