We narrowed to 136 results for: max

1. Custom CRISPR Screens & the Green Listed Software

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   Blog Post

   ...population level, but each individual cell only has a maximum of one gene knocked out. In this genetically heterogeneous...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

2. CRISPR 101: Cytosine Transversion Editors

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   ...UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for....2% of studied sequences) (Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower ...

3. 2022 and 2023: Reflections and Looking Forward

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   ...partners, and the larger scientific community to maximize the positive impact that science can have on the...

4. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

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   ...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

5. Management for Scientists: Seeking Feedback

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   ...gossip here – this means encouraging a culture of maximum information flow so everyone knows what is going...

6. 9 Tips to Achieve Success in Academia

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   ...academia How can we shape our time in academia to maximize productivity and success? We asked leading professors...

7. We're Updating Our Hold for Publication Policy

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   ...available as soon as your paper is published helps maximize the potential impact of your work. Plus, it’s ...

8. CASTing Off for New Shores in Human Genome Editing

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   ...natural evolution would produce transposons with sub-maximal efficiency, and this barrier could be overcome ...

9. Hot Plasmids and Viral Preps - July 2021

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   ...10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and minimize off-target effects...

10. Hot Plasmids - October 2022

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    ...potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm and two-photon excitation ...

11. Advice for Choosing a Research Project

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    Blog Post

    ...intelligent discussion, and/or brainstorming sessions to maximize productivity and direction of the project? What...

12. History of CRISPR Cas - A tale of survival and evolution

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    ...2011 Jun; 9, 467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432...

13. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post

    ...share your recombinant antibodies via Addgene to maximize the reach and impact of your work. Please email...

14. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...mammalian screens, which enables researchers to maximize the chance of transducing a only a single gRNA...

15. Pushing the Limits of DNA Assembly

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    ... of sgRNA. Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with...

16. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...need to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...

17. Viral Vectors 101: Types of viruses

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    ...discussed in this article, adenovirus has the highest maximum titer, a plus for production and infection. This...

18. Targeted Mutagenesis with EvolvR

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    ...special bacterial growth conditions in order to maximize the mutation rate. For example the Phage Assisted...

19. Communicating Your Science With Help From ComSciCon

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    ...audience about the work you've done. If you want to maximize the impact of your communication, you will also...

20. Dos & Don'ts When Publishing a Scientific Manuscript

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    ...contains all the necessary information to be of maximal use to the scientific communities. The ARRIVE guidelines...