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We narrowed to 210 results for: pcr

Showing: 61 - 80 of 210 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...primer sets to use to genotype your pups. A simple PCR with these primers will let you know if there are...are any major deletions, and sequencing of the PCR products will give specific details on what exactly ...alleles are present in these mice, you can clone the PCR products into a pBluescript cloning vector and sequence...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...use protocols that require restriction enzymes or PCR. The app contains a double digest tool that is only...Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence and the NEB polymerase...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...conjugates used in assays like proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020...(2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/...

  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...plagued by a steady barrage of problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there ... being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where ...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ... once decided that to teach high school students PCR, they first had to understand hydrogen bonds and ...unlikely to be able to follow a YouTube video on PCR, while a senior lab scientist is likely to be quickly...

  10. Tips for arabidopsis transformation

    Type
    Blog Post
    ... Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant takes...remaining starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once...

  12. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...

  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using...

  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic loci...and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ... technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9.... The plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag...expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ... be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...

  20. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited...
Showing: 61 - 80 of 210 results