We narrowed to 208 results for: pcr

...selection would only then kill the desired plants! PCR or fluorescent markers are currently used in these... construct could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive...

...primer sets to use to genotype your pups. A simple PCR with these primers will let you know if there are...are any major deletions, and sequencing of the PCR products will give specific details on what exactly ...alleles are present in these mice, you can clone the PCR products into a pBluescript cloning vector and sequence...

...use protocols that require restriction enzymes or PCR. The app contains a double digest tool that is only...Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence and the NEB polymerase...

...blogger post about colony PCR, I signed up immediately. I had used colony PCR before and thought it was...

...conjugates used in assays like proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020...(2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/...

... once decided that to teach high school students PCR, they first had to understand hydrogen bonds and ...unlikely to be able to follow a YouTube video on PCR, while a senior lab scientist is likely to be quickly...

...plagued by a steady barrage of problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there ... being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where ...

...perform PCR against components of the viral vector using the harvested viral solution as a PCR template...

...developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing. Figure 1: Overview...reversed so that DNA fragments can be detected by PCR or deep sequencing. A drawback of the original...

... Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant takes...remaining starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once...

...presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu inspire...

... use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...

...facility requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all...

... technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9.... The plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag...expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR...

...Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using...

... be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...

...differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic loci...and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral...

... AAV2 serotype, as titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers ...

...degrade the double helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of...

...procedure daily before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought...