We narrowed to 213 results for: pme i

... market, I am sure I missed some of your favorite apps. If you know of a software app that I missed, let...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked...grosses me out. Maybe it’s just me – I didn’t get a smart phone until after I had finished my PhD and left the...Addgene. I just began uploading our cloning protocols to the site and will keep you updated as I learn to... repository’s most frequently requested plasmids. I wondered how many scientists have used the protocol...used. After learning of it's continued popularity, I decided this protocol would be a worthy first contribution...contribution to the protocols.io community. Did I mention that this protocol is being used around the world...

...explained: “I took a microbiology class my sophomore year and it was the first time that I was in a lab...faculty members and it just blew me away….  I have to come here, I need to spend time here, this is an incredible... school at MIT. Doench excitedly remembers: “When I came to MIT to interview, part of the interview process... his PhD work in Sharp’s lab.  Doench says, “what I loved about [RNAi], ... it was just this complete ...be able to do and to organize it and catalogue it, I mean that’s the critical function really…. Addgene..., you need a tremendous amount of technology development alongside it.” Doench starting working with ... Doench says he enjoys working on technology development under the Platform model at the Broad Institute...

...some things can’t. I kiss my kids good night every evening I am home no matter how busy I am. Believe it ...lead a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and tactics...penicillin – I am much less worried knowing that she can take care of herself this way (except I am glad she...mental relaxation. And, I’m not sure I should put this in print, but I actually let my daughter skip school...organizations supporting women in science and career development. I try to do a really good job in one area at ...you are saying to yourself, “I’m not married,” or “I don’t have children, so my life will be under control...when they leave home which happens very, very fast! I will never forget the time my eight-year-old daughter...

...for Listeria in food products). I picked this post for a few reasons: 1) I think phage are super cool and...for Rare Disease, Running for FOP, Running for AJ I have two picks for the editor’s choice posts this ...could be useful for a wide variety of reasons. 3) I would love to see more posts like this on the Addgene...time we’re raising money for a cause while running. I love running and this event gives me the opportunity...September posts in chronological order here: Four Things I Learned at the 10 Years of iPSCs Symposium Tips for...Picking a Lab and a Project That’s it for now. As I indicated in the opening paragraph, I’m currently ...our attention and has huge potential for the development of therapeutics. It also represents a first for...

... community with their research, I think I can speak for Addgene when I say that I'm glad we're so fast...suggested that I might be able to attend the 10 Years of iPSCs Symposium earlier this year, I jumped at the...exposed to many scientific communities, but usually I only get to scratch the surface of different fields...conferences. In my nearly 20-year career in science, I have never attended a scientific meeting that hosted... daunting because they have no clear cut answers, I applaud the stem cell community for addressing these...is leading to promising clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who...questions of ethics can have profound impacts on the development of applications with the potential to save lives...

...When I first heard about it, I got excited from a computer scientist perspective. But, the more I thought...space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace is ...thought about it, the more I realized that it cuts across multiple disciplines, which really epitomizes ...in the Victoria area, please stop by. And if not, I suspect you can find a makerspace somewhere near you...counter movement by the public to access basic equipment and to become involved in developing tools and... skills to help do science on the cheap using equipment that would otherwise be discarded. For instance...learned to be resourceful and to repurpose old equipment for new uses. We’ll probably never use the Illumina...

... MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started working...that I learned about from textbooks just made everything click in my head and left me thinking: I wish...in my first molecular biology lab in college that I really fell in love with it. Something about being...wish I had this kind of experience earlier! After all, students build circuits in physics class to learn...Unfortunately, having live cells in a classroom presents equipment needs, such as shakers and incubators, and biosafety...Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time plasmids ...

...Director of Science Product Management “I knew very early on that I wanted to be an artist/designer. But ..."not a real job," and that I should pursue a real career. In art school, I came across Joseph Campbell...your bliss' mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting...getting into the arts (or any career to be honest), I would tell them to follow your passion. Do what you...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...

...capabilities. In just two years as a healthcare consultant, I have worked on most of these kinds of projects. Almost... must to be successful in this field. Personally, I have greatly benefited from and appreciated the training...economics, or in some combination of these fields. I got my PhD a couple of years ago in Bioengineering...critical analyses skills were applicable to my cases. I can directly use my understanding of the molecular...pulling your weight with your scientific expertise. Do I have to stick with consulting in the long-term? A ...be a great fit for former healthcare consultants. I have even seen consultants go back to research and...academic lab. How to get your foot in the door When I was first considering preparing for and transitioning...

...Administration, I could never get one straight answer about what science policy even is! In this article, I hope .... “But, seriously, what even is science policy?”  I have been asked this many times throughout my short...from academia is the uncertainty of it all. Though I had spent the first few decades of my life following...less than two years of walking away from the bench, I have worked for seven different organizations in a...and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy ...local level. The big secret about science policy that I didn’t learn until arriving here in DC, is that so... to get involved.  Why do you do science policy? I would argue that this is the most important question...

...tubes and gels). As a freshman in college I studied development and evolution of histone genes in Strongylocentrotus...tank, and observe the beauty of embryo development in real time. I was already a biology geek, but this ...nailed down my plan to become a research scientist. I recently was invited to attend the first Principal...Enabling Discovery Through Genomic Tools) Program and I met scientists from 21 different labs who clearly ...educational programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ...important models for solutions to the coral reef crisis. I like how they are using protocols.io to create a protocol...studied over time. They recognize individual faces (I guess of other wasps) so can be used to study aspects...

... are collaborating on projects. When I was a graduate student, I led a collaborative project between my...specialized equipment, or ensure that techniques are being performed the same way at different sites. I did a... a lab exchange as part of the collaboration I led. This gave me access to specialized microscopes and...interest in a French clinical cohort, neither of which I would have been able to do from my home lab. Scientific...collaborations are becoming more and more common. I hope these tips are useful whether you’re looking ...

...people, I quickly realize that a company requires more than just good data to become a product. I spoke ... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...impact on healthcare and the community. As a result, I have occasionally pondered translating my research...insight into "the random-walk" of a budding startup, I asked Mike to outline 3 pieces that are essential ...Koeris, co-founder and current VP of Business Development & Operations of Sample6, for advice on how to...months in graduate school focusing on concept development and participating in business competitions. When... COO, then moved on to become VP of Business Development & Operations. Starting a biotech company ...

...you can say “I usually need time to gather my thoughts, but always have questions once I do – would it... more generally “How am I doing? Can you tell me what’s going well and what I could improve on?” Having... send me the protocol we’ll be using next week so I can familiarize myself with it.” It won’t be a bother...can you remember everything? Write it all down! And I mean everything! The bare minimum should be taking...have time, would you mind helping me with….” vs. “I need help with…now”. Ask for feedback so you can ...adjustments. This could look like “What are some things I could pay attention to, so my dissections go smoother...Visiting Scientist Edition Mentoring for Scientists: I Have a Mentor, What Now? Will You Be My Mentor? Finding...

...It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal ...retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated ...degenerative disease called Retinitis Pigmentosa. I quickly became fascinated by this emerging technology...potential for the treatment of some genetic diseases. I didn’t know it at the time, but gene therapy had fallen...s congenital amaurosis. This is the first of what I, scientists, doctors and patients hope are many more...29868583. PubMed Central PMCID: PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 ...being benched due to the high cost of clinical development. As a result, Luk Vandenberghe and colleagues...

...academic postdoc. Why did I switch? Well, my long-term goal was to enter industry since I was drawn to the idea...grant-writing. The more I talked to people with industry experience, the more I heard that getting into...Desrochers, a postdoctoral scientist at AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry...major life decision, the transition felt scary. While I knew two people from graduate school who were at AstraZeneca...assure me that they were having a positive experience, I was still incredibly nervous about leaving academia...will also have to attend many more meetings. How do I get an industry postdoc? Many companies have industry...AstraZeneca. She has a background in cell and developmental biology and is applying this towards researching...

...Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants, but the seeds needed...tissue for imaging or collection could contaminate it. I have used it for selecting transformation events and...Zhang, T., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the ...RUBY and the construct that rescues the mutation. I will be able to easily choose the green seedlings ...these can be challenging and requires specialized equipment. Reporters for studying gene expression have similar...Willoughby is a graduate student studying plant development and cell-cell communication in the Nimchuk Lab...

...of the developer and I get to see an approach that is likely different from what I would have done. And... for open science. I hope you enjoyed learning about Addgene’s software development team and how we work...developer. This is another point of collaboration for us. I enjoy doing code reviews because it’s like a little...take turns working on different parts of the code. I think the best way to learn about how any code works...software development happening at Addgene under the hood. A significant portion of our development may not...productivity. Get to know our software development team and the development process at Addgene. Diversity supports...When you think of Addgene, chances are software development is not one of the things that comes to mind. ...

...’s. I was facing the prospect of having to do a third postdoc in order to rebuild my resume, so I had ...laboratory lease, etc. I still flip to that page in my lab notebook from time to time. I wrote in large letters...often along the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was...in a cutting-edge synthetic biology lab Keira and I worked in the same lab for four years. It was a cutting-edge...cutting-edge synthetic biology lab focused on product development: building a plant that could sense and respond...

... et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency...the model organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows...CRISPR editing in C. elegans improves efficiency  I was fortunate – or unfortunate enough, depending on... alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...courtesy of Jordan Ward, UCSF. In 2015 (Ward, 2015), I demonstrated that selection for repair of a temperature-sensitive...Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal...selective marker to remain in the edited animal. Development of CRISPR/Cas9 editing strategies in C. elegans...