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We narrowed to 393 results for: prep

Showing: 61 - 80 of 393 results

  1. Viral Prep Service for Plasmids and Pooled Libraries

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    Collection
    ...started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...Service Addgene produces high-quality viral vector preps from select plasmids in our collection. We know ...high-titer preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo...high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse...Request Can't find what you need in our in-stock preps? Our Packaged on Request service offers you even...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging...

  4. Plasmids 101: Colony PCR

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    Blog Post
    ... cultures with the clones you pick and only mini-prep the positive ones. Regardless of which method you... a few positive clones, the last step is to mini-prep these clones and submit the plasmids for Sanger ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...

  5. AAV Packaged on Request

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    Collection
    ...service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification...protection levels. We offer 963 in-stock viral vector preps, delivering high quality and ready availability ... many researchers, scientists sometimes require preps beyond our current in-stock capabilities. To meet...start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce,...All-in-One Pricing The price for our Packaged on Request preps is all-inclusive of several important steps: MTA... varies by requesting organization Viral vector preps remain subject to the terms and conditions of the...priority. During this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes...

  6. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...eliminate any HEK-293T cells. If you titrate your viral preps, lentiviral titration requires an additional couple...Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols...transfection). This differs from typical insect cell preparations, which we talk about below. Processing The viral...

  7. How to Be an Excellent Trainee

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    Blog Post
    ...In research there is a saying that if the sample prep is poor, then the data will be poor. The same is...assistants, and sitting out on the bench waiting for you, prep for research work has to be done by the researchers...highlight how to prepare and things to keep an eye out for during training. Preparation before bench work...the training. In this blog we will review the preparation and planning you can do to build a bench mentor-mentee... training in the lab! If you don’t do proper preparation and pay attention during training, then you won... you – so do your best to show up as your most prepared, can-do self and make the most of it! So, what... schedule, be respectful of theirs! Show up prepared and take training seriously, as reviewed above...

  8. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment...Great news. A subset of our collection has been prepared as part of our new cloning grade DNA service and...plasmid without spending time and resources in preparative experiments.  We’re excited to begin offering...etc. and/or directly long term storage. Every preparation is quality controlled through next generation...

  9. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...experimental details on the spreadsheet: Name your viral prep Enter the specific names of the plasmids you plan...goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help ...

  10. AAV Viral Preps

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    Collection
    ... AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps are purified...and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified ...reported with each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request ... pages above. You can also do a search of viral preps and filter by the serotype needed....

  12. Newly Updated AAV Data Hub!

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    Blog Post
    ...submissions will receive a 20% off coupon for a viral prep. And if you've used the Data Hub to help select ...

  13. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org: Check out the lentivirus...lentivirus guide Find ready-to-use lentiviral preps at Addgene ...expression using other methods.  In terms of preparation, VSV-G is stable and can withstand ultracentrifugation...

  14. Addgene's Website Update

    Type
    Blog Post
    ...Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those are the biggest...

  15. Lentiviral Prep Service

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    Collection
    ...plasmid from us or receive a ready-to-use viral preparation from the inventory below. Lentiviruses are distributed...

  16. Biosensor AAV Preps

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  17. Chemogenetics AAV Preps

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    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...Sternson 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent...* Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent...

  18. Control AAV Preps

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  19. Optogenetics AAV Preps

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...table and filters below to find ready-to-use AAV preparations of optogenetics plasmids available in Addgene's...

  20. Recombinases AAV Preps

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...AAV Browse the following tables for in-stock AAV preps that encode site-specific recombinases, which can...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
Showing: 61 - 80 of 393 results