We narrowed to 110 results for: quo;

1. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

2. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just part of...

3. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   ...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

4. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...freeze-thaw cycles and should be stored in single-use aliquots. Never use a polybrene solution that has been ...

5. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ...long term use, you may want to consider using an aliquot that has been subjected to a freeze/thaw cycle ...

6. 10 Basic tips for mammalian cell culture

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   Blog Post

   ...starting off with a new cell line, grow the cells, aliquot them into a number of vials, freeze them, and store...

7. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

8. Pooled Library Amplifications

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   Blog Post

   ...plasmid diversity, so Addgene strives to distribute aliquots of the original library whenever possible. In ...

9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

   ...superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an...

10. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...Fluorescent Protein (GFP), a protein from the jellyfish Aequorea Victoria. Since that discovery, the family of ...

11. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ... from where you prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and ...

12. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing buffer, polymerase...

13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...amino acids derived from the crystal jellyfish Aequorea victoria. It has a fluorescent emission wavelength...

14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ... all the bins together at a mixer. We call this Quorum Sensing, after the phenomenon in bacteria that ...

15. An Inside Look into Addgene's Dev Team

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    Blog Post

    ...and in other places, we might be calling it an aliquot. This can lead to confusion as to what the code...

16. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ... Figure 2: Label that comes on Addgene’s AAV aliquots. Our titer values (circled in red) report physical...

17. How to Write a Scientific Review Article

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    Blog Post

    ...challenging. My best advice is don’t overthink it. And to quote my adviser, "you just gotta do it." At the end of...

18. Getting the Most from Your Lentiviral Transduction

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    Blog Post

    ... viral preps should be divided into single-use aliquots, and stored at -80°C. Some reports suggest rapidly...

19. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...referring to the evil side of a mystical "Force," this quote is equally applicable to many microscopy experiments...

20. March for Science

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    Blog Post

    ...it happen. In a time when a top official can be quoted as saying “I’m not in the job of having evidence...