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New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

CRISPR is a story of revolutionary science - but it's also a story of community and sharing. Addgene shares our part in this story.

If you're designing a flow panel with a large number of markers, it might be time to look into spectral cytometry instead of conventional flow.

There are many ways to communicate your science - so here we're covering how to pick the best one for you.

Learn how to identify negotiation skills you already use in your lab and life and develop them further as a professional skill set.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Drew Endy discusses the Biobrick Public Agreement (BPA) Plasmid Collection, BIL gates, BIOFAB, and the future of the bioeconomy.

Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette.

Addgene co-founder Melina Fan is transitioning to our Chief Scientific Advisor. We reflect on our history, how science has changed, and what comes next.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

Learn how you can identify your transferable skills and better prepare yourself for your next job interview.

Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, provides tips on getting a faculty positions & fulfilling your academic career goals.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.