...is the right way?  First of all, I want to put to rest a common misconception. I find personal bias often...If you're interested in sharing your science, one of the big questions you'll be faced with is "how?"...be able to access your content.  What are you interested in? Math, sarcasm, romance, and spacecraft...can guarantee you that either resource or time restrictions (or both!) will be a major limiting factor at... to reach people who are not already actively interested in scicomm. Difficult to do in-depth storytelling...

...minutes of the two hour time slot, so she can take the rest of the time to collect her data. Perhaps you both...

...protein-coding genes, and you might be wondering what the rest of our genome could possibly be made up of. The ...options. By carefully considering your ncRNA of interest, you should be able to devise an experiment that...

...CRISPR tools were committed to sharing them with the rest of the community. They did this by depositing them...

...genome scale, it's going to be tricky to tame the rest of a cell. The BIOFAB plasmids represent the first...

...recommend that you plate 50 μL on one plate and the rest on a second plate. This gives the best chance of...also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation... how to transform E. coli with your plasmid of interest. Protocols...

...you even wear a lab coat! These are the things the rest of the world imagines when they think “scientist...your job as well as possible. It’s in their best interest to do so, sure, but it also eliminates that feeling...

...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...CLAP) with a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling...studies of kinesins but for many other proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! ...

...sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing and...This score is a useful measure for those who are interested in understanding how many of the contributing...

...part time work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer...Support and Operations Manager at Addgene and is interested in nonprofit management and international development...

...of the undesired portion, designed to amplify the rest of the vector sequence. Encode homology in the forward... longer oligos, which could be avoided using restriction enzyme cloning, were more prohibitive at the ... doesn’t matter if the DNA is PCR amplified, restriction digested or even a synthesized gene. PCR is the...IVA can be employed by cutting the vector with restriction enzymes and adding the corresponding homologous...the MRC Laboratory of Molecular Biology. He is interested in neuronal signal transduction, and uses electron...

...AMA Managing time and energy between work and the rest of life is sometimes more of a struggle and sometimes...negotiating a demanding career in science with other interests and priorities, but many find it helpful to obtain...

... that (1) the sequence is unique compared to the rest of the genome and (2) the sequence is located just...

...where you are going and what you expect. For the rest of the chalk talk, as with your job talk, avoid ...and tissues is fundamental to development. I'm interested in how cells find their way to the correct positions...expert in [your research area]," – you should show interest in other faculty members’ research. Still a Grad...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

...conditions:  1) The sequence is unique compared to the rest of the genome.  2) The target is present immediately...Cas9-mediated frameshift mutations in the gene of interest. The genomic target for Cas9 and the introduction...site early in the coding region of the gene of interest. This gives you loads of sequences to select from...to find a SpCas9 compatible PAM at your site of interest, that is the most efficient option. If there is...repressors to the promoter region of the gene of interest. dCas9-based repressors are useful when knocking...

.... Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not...establish cell lines that stably express a gene of interest. While the general approach for using lentivirus...the positively charged polycation, polybrene. Interestingly, one study found that alternative polycations...

...release the plunger, allowing it to go back to its resting position. Wait for the liquid to stop flowing into...

...protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction enzymes...relatively well understood biology to answer many interesting questions. Does my transcription factor bind ...and/or alter cellular activity in a way that’s interesting from an applied perspective, sometimes it’s best...Plasmids Tn7 Transposition Nancy Craig A gene of interest cloned into one of these plasmids can be transposed... many enterobacterial genomes. The bacteria of interest are transformed with the appropriate construct...get a better understanding of what your gene of interest does when expressed in vivo . Plasmid ID Visualization...fluorescent protein fusions with your protein of interest. See an overview of Addgene fluorescent protein...

...protein of interest. Signal strength is determined by how many antibodies bind the protein of interest, while... antibody of interest to a second, independent antibody for the same protein of interest, with no overlapping...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called... of the animal’s immune system. The antigen of interest is introduced to the animal, which then mounts...primary antibody which is bound to your protein of interest. Secondary antibodies can be isotype-specific ...recognize an available epitope on your protein of interest. Polyclonal antibodies are typically collected...Western blots should be run with your protein of interest and at least one ubiquitously expressed protein...

... of interest fused to the N-terminus of Amber Amber C1 Yellow Mammalian Express a gene of interest fused...a fluorescent fusion protein with your gene of interest and the listed fluorescent protein. Plasmid Color... mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean ... mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean ...His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow...Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the N-terminus of monomeric Venus mVenus...mVenus C1 Yellow Mammalian Express a gene of interest fused to the C-terminus of monomeric Venus pCEP4YPet-MAMM...