Plasmids 101: Restriction Cloning
Type
Blog Post
Published
Feb. 18, 2016, 3:42 p.m.
...When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone... of how to do perform restriction digests.
Overview of the restriction cloning process. Both ...) and the DNA sequence of interest (green, insert) are cut with restriction enzymes to generate compatible...backbone will contain a variety of restriction enzyme cut sites (restriction sites) downstream of the promoter...DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently...backbone covalent.
Before beginning the restriction digest and ligation process, you should carefully...these both must have compatible cut sites for restriction enzymes that allow your insert to be placed into...