We narrowed to 216 results for: rras

...the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes...

... from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription...

...neurotransmission. These receptors can be activated by an array of different ligands, including hormones, neurotransmitters... showing no constitutive activity. There are an array of DREADDs that activate different G proteins, work...

...guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image...

...successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who have had a ...offices or professional organizations can provide referrals. There are bad coaches out there, so finding a...

...bright and stable fluorescence of the original (Ivorra-Molla et al., 2023). They also named their variant...: https://doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold ...

... the (1) binding of calcium, (2) allosteric rearrangements of the sensor, and (3) subsequent change in... with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous calmodulin...

..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...

...into two categories: probes and editors. A probe array may consist of at least two sensor modules targeting...via CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags, including...

...post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter ...  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University College...

...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038...

...-020-00361-z Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration...https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J.,...

...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution...

...later modified by others to produce Murray’s clear solution.  Murray's clear (11) was mostly used to study...

...single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations Making...simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon...

... that may have warranted their own chapters, and certainly chapters that could warrant their own books...

...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors..., Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, Maouche-Chrétien L, Bernaudin F, Girot R, Dorazio...

...Cpf1 multiplexing approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest...Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID...

...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations...

... the lab. GFP is used in research across a vast array of biological disciplines and scientists employ ...maturation, and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed...