We narrowed to 91 results for: sas;

1. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

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   ...    We have GIFs for every occasion. Disaster in the lab you haven’t quite gotten over? We have...

2. 2018 #DeckTheLab Contest Winners

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   ...Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ pic.twitter.com/X6QYXteRLT...

3. What's New in CRISPR - Spring 2019

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   ...Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but preferentially...

4. Bringing Sustainable Practices to the Lab: Innovations

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   ... which we now realize is a major environmental disaster. We should continue to examine our behavior and...

5. Antibodies 101: Fc Effector Functions

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   ...don’t generally affect antibody applications like ELISAs or Western blots, they are key in helping antibodies...

6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   ...advantage but error-prone repair of the gene would be disastrous (i.e. if breaking the gene is worse than leaving...

7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ... Lastly, once a mutant is identified, the PB transposase can easily and seamlessly remove the integrated...

8. Viral Vectors 101: Viruses as Biological Tools

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   ...inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate...

9. Antibodies 101: Isotypes

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   ...cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting.  The five isotypes of antibodies...

10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...densely populated places like the University of Kansas and the University of Louisville in Kentucky, the...

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity to light, and better...

12. Cloning Mammalian Cells with the Agarose Method

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    ...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing...

13. Plasmids 101: Secondary Nanobody Toolbox

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    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...

14. Neuronal labeling with Spaghetti Monster

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    ...design in public schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon...

15. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

16. Hot Plasmids - August 2020

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    ...into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker et al., 2020. The lab...

17. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat. Biotechnol. 2020 387...

18. CRISPR Activation: A Practical Guide

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    ...Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae...

19. Antibodies 101: Beyond Surface Labeling

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    ...system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling employs...

20. Five Popular Model Organisms, Part 2

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    ...including plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus Xenopus, or frogs...