We narrowed to 100 results for: sgRNA

Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids.

Find some tips and tricks for using CRISPR for gene editing.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

Laura Drepanos from the Broad Institute shares the strategies her team used to optimize the CRISPR knockout libraries Jacquere and Julianna.

CRISPR/Cas9 systems have been shown to mediate gene correction in mice and in human stem cells demonstrating the therapeutic potential of CRISPR technology.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems.

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Learn about the latest CRISPR plasmids deposited at Addgene, including a miniature CRISPR-Cas systems, SEND plasmids, and prime editing improvements

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

CRISPR plasmids for endogenous tagging of your gene of interest.