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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 130 results for: tac promoter

Showing: 61 - 80 of 130 results

  1. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...one of the most common criteria required for a promotion. Yet little explanation is given for how someone...the work gets done Managers do this by removing obstacles that stand in the way of the doers and by ensuring...

  2. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...You will have the option to indicate separate promoters, tags, or cloning information for each insert.... 8: Add vector backbone information. List any promoters, cloning details for how your gene was inserted...greater than 75 plasmids, please let us know by contacting [email protected]. These deposits are considered...us at  [email protected] Special cases Please contact us at [email protected] or +1 (617)-ADDGENE (...deposit a pooled DNA library with Addgene?" or contact us at [email protected] or +1 (617)-ADDGENE (...genomic alterations unique to your strain. Please contact us at [email protected] or +1 (617)-ADDGENE (...

  3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and ...Research tackling questions in the fields of Cancer, Inflammation and Immunity, as well as various combinations...Researchers and Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary.... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few...are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...d like to share with the scientific community, contact [email protected] to find out more about our ...

  4. RUBY-Red Siliques

    Type
    Blog Post
    ...of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator... to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity of ...

  5. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...specific times or locations using cell-specific promoters or inducible systems, you can precisely control...fragments and placed under the control of different promoters. Expression of both fragments in the same cell... lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs...plasmids based on your expression system of choice, promoter, or search by inducible system (e.g., search for...Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Explore our full...Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search our full...

  6. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...released into the environment. Various factors promote natural transformation in different bacteria such... or sex pilus that attaches to the recipient cell drawing it close. Once in contact the donor can transfer...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...bacterium to a recipient bacterium through direct contact. The donor bacterium contains a DNA sequence called...

  7. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... and offer congratulations on job changes and promotions.  Follow academic scientists on ResearchGate ...would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists... level of training is always a good idea. Make contact with other attendees in advance. Join a LinkedIn...

  8. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...straightforward to define, but for control elements such as promoters and transcription terminators, the boundaries ... common feature detection in a new way, please contact us at snapgene.com. Many thanks to our guest bloggers...

  10. mTOR Pathway

    Type
    Collection
    ...anabolic to catabolic processes. Active mTORC1 promotes protein and lipid synthesis, and increases energy... cancer. Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial...mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions needed for uncontrolled cell growth... which appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway ...Cancer Pathway ORF Kit Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening ...Resources Cancer Pathway ORF Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening ...

  11. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...was shown that putting the operon under a strong promoter results in high R-body expression  (Schrallhammer...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since...

  14. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...studying how that one protein interacts with that one promoter can tell you, it can be easy to get lost in the...factors). As the Yamanaka lab's primary Addgene contact for many years, I've been looking forward to seeing...

  16. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ... gender analysis for innovation and discovery. Tackling sex and gender in research Governments, universities...

  17. Hot Plasmids - October 2020

    Type
    Blog Post
    ...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

  18. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...a requirement. Your manager: Unlike academia, promotions in industry are based on people’s ability to ...which means you’re more likely to have the right contacts and get your resume pulled to the top of the pile...position. Work your LinkedIn connections and other contacts to get that referral and find out more about the... postdoctoral experience Read Joanne Kamen's 8 tactics for choosing a lab Resources on Addgene.org Find...
Showing: 61 - 80 of 130 results