...vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids. For example...plasmid and the recombined vector backbone, you can try re-streaking for single colonies and testing multiple...

...well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique....these tips are helpful to you no matter what you’re trying to accomplish during this period. ...

...researcher decides on a new project and begins by trying to reproduce someone else’s result. Then, they ...that they thought would be moving forward instead trying just to get going. It’s like being stuck in jail...

... enough - but this week we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday...

...from Ma et al., 2018 with permission.   Why try CRISPR-Sirius? With all the tools out there to visualize...visualize genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References...

....  We hope you will give our new search engine a try to find reagents and educational material. Let us...us know what works for you and what doesn’t. Try out the new Addgene search engine! Additional resources...

...Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the pledge to have... being an educated mentee. Other opportunities: Try virtual mentoring at Mentornet.net. This is an online...seeing a lot of success at universities around the country with groups who are piloting this. Watch for my...

...occasional errors. That can be advantageous if you are trying to knock out a gene. One can also exploit homology-directed...induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have the mutations...

...RNA structure and the site in the genome you are trying to target play a large role in Cas9 activity and... CRISPR mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir...homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722...

...molecular cloning. Depending on whether you are trying to overexpress or knockdown a gene, you might need...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...how the scavenger hunt works works: You will be trying to find pictures of Addgene pets with Addgene-branded...

...send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone app either as ...suit, but this is not Facebook so no pets or kids. Try looking a bit over your shoulder for the picture....

...nitrocellulose, you may decide you have nothing to lose by trying to strip and reprobe. In this case, we recommend...procedure, perhaps using longer incubation times, or try the stringent solution if you started with the mild...

...during protein folding, in which case you should try tagging the opposite end.  If neither placement works...protein of interest is expressed at a very low level, try a longer histidine tag to increase capture. Longer...

...that blue and violet light can penetrate. How can I try using photoswitchable kinases in my experiments? ...into loops of a protein of interest. If you plan to try this, it’s important to check that the function of...

... you. When embarking on a new literature search, try to find a recent review article to give you an overview...you are looking for a particular protocol or are trying to replicate an experiment, the methods section... you to our guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences...photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter @JLRoose....

...gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip...

...summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were the plasmid...

...not doing yourself or your science any favors by trying to go it alone. The help of fellow lab members .... If I invite someone to be on a project team, I try to make it very clear why that person is there. If...thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem...

... get a lot of office politics (many individuals trying to divert more resources towards their projects...modern genetics from the perspective of somebody trying to get hands-on lab experience is that the technology... may seem quite unusual but it has allowed me to try out working in many different sectors and to discover...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...months experience working in a developing tropical country. This is why I set up a placement working in a ...