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We narrowed to 1,010 results for: RING;

Showing: 391 - 400 of 1010 results

  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...job. How can you incorporate the skills developed during graduate school into your resume? How can you stand...Here are a few things to consider when you're preparing your resume for a non-academic position: What ... current job as a Communications Coordinator. Wondering how you can develop more transferable skills? ...? See Developing Transferable Skills During Science Training and So You Think You have Skills.   Get Science...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,...

  2. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...components are still there in real life.  Certainly referring to any DNA within the context of a nucleus as ...methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the level...! And if you study chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to...Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander Stark Bas van Steensel Richard Young...chromatin and the epigenome. If you have been considering some new techniques, the posts below may steer...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...” has me considering the microbiome more often than not). If you’re someone who’s considering getting ...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...because they provide a highly versatile means of delivering genes to many different cell types with minimal...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

  4. Clear and Accessible Writing

    Type
    Blog Post
    ...accessibility is removing barriers when we can and lowering them when we can’t. We know barriers exist in ...layers are blurred after centrifugation and the syringe needle is inserted bevel-up at the interface between...the 40% layer. To collect purified AAV, insert a syringe at the interface of the 40% layer containing the... iodixanol layer, with the bevel pointed up. A syringe at the top of the tube allows air to enter the ...the column so the virus will flow out the bottom syringe."Feel free to share your alt text in the comments...

  5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...interest in sharing, there will always be an interest in protecting intellectual property. Figuring out that...of Katz and Paratore onto the Board of Directors brings complementary experience and insight to the now... to the scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...Internal Audit function and is responsible for bringing a systematic and disciplined approach to evaluating...

  6. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social...conference, whether you are attending in person or monitoring that conference via social media. The podcast...Twitter is a tool worth using, but you might be wondering how to get started. This blog post outlines 10...unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler on ...

  7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...off-target cleavage should be an important consideration during any experiment. Sensitivity Cas9 sensitivity is... the Cas9 nuclease. This can be determined by measuring the indel frequency at a target site. A specificity...not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...PMCID: PMC6368452. Strecker, Jonathan, et al. "Engineering of CRISPR-Cas12b for human genome editing." Nature...Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018): 63. PubMed PMID:...

  8. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...genome replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and...restriction enzymes are one half of naturally occurring restriction modification systems that prokaryotes...DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired mutation...methylation Finally, you can control methylation by altering your choice of bacteria. For example, if you must...

  9. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...most important step in ensuring quality, as it will catch any errors made during the cloning, sample handling...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...samples coming through our doors, the first step to ensuring plasmid quality is to stay organized. Here at ..., antibiotic resistance, restriction sites used during cloning, and most importantly, the predicted full...

  10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...retinal ganglion. Rather than using naturally occurring promoters to drive transgene expression, the Roska...one type of cell, with the remaining expression occurring in only Müller glia cells, a common contamination...retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in mouse, monkey...targeted cells in monkey and human retinas. By comparing the performance of promoters across these three...be paired for targeted expression. This same pairing of synthetic promoters were also used to target...
Showing: 391 - 400 of 1010 results