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TypeBlog Post...and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very...Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR to Quickly...
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Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post... of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins...proteins. Instead, consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus...investigate transcription factor candidates and the regulation of genes in the biosynthetic pathway. Why plant... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...applications, they have become indispensable tools for manipulating cells and organisms. They can be used for a ...This is because most titering methods rely on isolating genomic DNA from transduced cells and quantifying... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...different opinions, and some of them would acknowledge later that without my “neutral” presence to alleviate ... that nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... Originally reported as a monomer16, IFP2.0 was later found to be a dimer3,15. ND-not determined in parallel... -
Evolution of Lab Techniques
TypeBlog Post... require sequencing of many samples at once for later comparison. These next-generation sequencing techniques...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...super-resolution microscopy techniques, such as Stimulated Emission Depletion (STED), and Stochastic Optical... expand the self-labeling capabilities of this platform. The Janelia Fluor ligands provide brighter and... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...replaces a target region with a newly synthesized, templated sequence. And the story still isn’t over! Keep...Base Editor A base editor that relies on DNA glycosylation to create an abasic site. In this post, it refers... -
New Antibody Animation!
TypeBlog Post...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ... -
New Coomassie Protocol Video
TypeBlog Post...determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant... -
New Antibodies in the Lab Animation!
TypeBlog Post...where to start or what your options are? Addgene's latest animation is here to help! "Antibodies 101: Antibodies... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...We are very excited to share the latest edition of our Viral Vectors 101 eBook! This practical guide... -
Antibodies 101 Series on TikTok
TypeBlog Post...whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ... -
A Case Study of Addgene Bioinformatics
TypeBlog Post..., they break down the unique challenges of our platform and how we were able to address these issues using... -
'Tis the Season to #DeckTheLab
TypeBlog Post...the month of December are eligible to win a hot chocolate party that will warm up the entire lab. We’ll ... -
The 12 Days of CRISPR: 2021
TypeBlog Post...to me: enhanced prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers: Atharva Sahasrabudhe... -
New FLIS Plasmid (April Fools 2022)
TypeBlog Post...rather astonishing ability to...well, annoy. Fly-related Issues in the Lab A significant drawback of working...