We narrowed to 885 results for: TRI

1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...

2. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...these systems in your research? Cary Valley contributed to updating this article. References Cox, David...

3. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...with the desired fluorophores. In figure 4B below, triple colocalization is possible even when staining cells...

4. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ... students work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...immediately after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period...

6. A Guide to Starting Your Own Journal Club

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   ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...

7. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ...viral genome (Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must ...

8. CRISPR Antimicrobials

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   ...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving CRISPR...

9. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   .... Lowered expression levels will increase the stringency of your binding. And most importantly: Independently...

10. Plasmids 101: Protein tags

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    Blog Post

    ...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...

11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    .... APOBEC-Cas9n-UGI targets its preferred trinucleotide motifs (5’-TCA-to-TUA) found within the 43 base-pair...

12. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

    ...and its subsequent repair. Additionally, the distribution of enrichment and depletion scores for safe-...

13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...and Frasch, 1993).  Tinman, which is spatially restricted to the dorsal mesoderm, is necessary to activate...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...vivo assays in which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far...

15. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...that this system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It...

16. Hot Plasmids: Fall 2024

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    Blog Post

    ... & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency...

17. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ...of flow cytometry is to use increasingly more stringent markers to identify your specific cell population...

18. Plasmids 101: Codon usage bias

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    Blog Post

    ... cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures...

19. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

    ...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold...

20. Tips for CRISPR Gene Editing in Mice

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    Blog Post

    ... This post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has...