We narrowed to 954 results for: COMP

...sufficient to give specificity and to also be compatible with a standard PCR reaction (see PCR Video)....design is similar, but we need to use the reverse complement to get PCR amplification. We can start similarly...the ORF). We now need to generate the reverse-complement of this sequence so that we can successfully ...amplify the ORF. You can generate the reverse-complement using existing software (a quick internet search... out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research...

...Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with CRISPR ... the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation or ...customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013)....appealing since the RNA scaffold modules are comparatively small allowing multiple sgRNA constructs to .... (2013). PubMed. Zalatan, J. G. Engineering Complex Synthetic Transcriptional Programs with CRISPR ...

...1); similar to an MRI or CT scanner. This is accomplished by focusing excitation light to a point in the... bleaching) to a single focal point. This is accomplished by using, for example, red light instead of ... photons of red light are needed to excite GFP compared to only one of blue. Only at the focal point of...coverslip and a sample in aqueous buffer it is completely reflected. This reflection occurs due to the ...immersed in. Although the excitation light is completely reflected, energy is propagated into the sample...cover samples of this size, those that can will completely image large tissues in a few hours. Although ...

...downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...immune response to the development of tumors is a complicated and dynamic phenomenon that is interestingly ...rear its head once the body’s immune system is compromised either due to age, infections, or other strenuous...focus on include: Cell heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity...successful tumor progression. A tumor’s genetic composition is constantly in flux and is dependent on the...

... other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm...SYBRGreen, are specific for double strand DNA (as compared to spectrophotometric methods that measure all... corresponding fluorescence. That way, you can compare the fluorescence of your sample against this curve...relative to the intensity in the ladder. It’s best to compare the band intensity of the fragment in the ladder...sugars under acidic conditions and forms a blue complex that can be quantified at 595 nm. This method is...

...commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as pichia). What...general research community. Phillips Petroleum Company first commercially developed K. phaffii in the ...Since then, multiple biotech and pharmaceutical companies (along with some research laboratories) have picked...of the uses outlined below. Biology They say comparison is the thief of joy, but I think it’s acceptable...10.3389/fmicb.2020.607028  Riley, R., et al., Comparative genomics of biotechnologically important yeasts...

... precision gene editing. CRISPR-Cas9, the key component to enable efficient base editing and prime editing...unable to process its own array but is the key component to enable efficient base editing and prime editing... or any other kinds of CRISPR tools, without compromising their strategic advantages in gene editing, ...higher Cas9-independent off-target editing in comparison with single-site editing. Encouraged by the results...#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

...equivalent of crossing off days on the calendar after completing your writing goal, which can serve as positive...graph of which days of the week you most often completed your habit is useful for figuring out when to ...makes suggestions on how to make your writing less complex. While I don’t think you should accept all of the...jargon in your writing. While it’s not possible to completely avoid jargon, this tool can help you avoid overusing...collaborators. Zotero is a desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero...

... potential binding affinity to major histocompatibility complexes (MHC class I and II).  There are multiple...of adverse reactions In silico assessment uses computer modeling and predictive algorithms to forecast...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ...in-vitro models can be built that contain various components of the immune system. Figure 2: In-vitro...

...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...Most of our equipment has been donated by local companies and BosLab has grown into an organization consisting... Timothy Stiles joined BosLab last year with a computer science degree and had zero pipetting experience...yeast to glow, produce synthetic scents, detect compounds in the environment, and openly publish protocols...the local community (including local garden and compost clubs) to participate in this study. How to get...