Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,010 results for: RING;

Showing: 401 - 410 of 1010 results

  1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...made of protein-coding genes, and you might be wondering what the rest of our genome could possibly be ... modulate ncRNA expression without permanently altering the DNA, CRISPR interference (CRISPRi) and CRISPR...high specificity, particularly in applications requiring smaller delivery systems (Zibitt et al. 2021).... a number of different options. By carefully considering your ncRNA of interest, you should be able to...RNA-targeting CRISPR enzymes for transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer...

  2. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...retinal ganglion. Rather than using naturally occurring promoters to drive transgene expression, the Roska...one type of cell, with the remaining expression occurring in only Müller glia cells, a common contamination...retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in mouse, monkey...targeted cells in monkey and human retinas. By comparing the performance of promoters across these three...be paired for targeted expression. This same pairing of synthetic promoters were also used to target...

  3. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...their promises, Wet-blanket negativist, who can bring down the morale of a group with their pessimism,...communicate their ideas confidently while also considering the needs of other people. Some students and ...Regardless of your supervisor’s management style, bring challenges to his or her attention as soon as you...signed, or manuscripts that need to be reviewed, bring them to the meeting. This will make it easy for ...problem by stating the facts and explain how it is interfering with your work. Avoid talking about your emotions...

  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...perform functional tests. The newest genome engineer sharing the stage with much-lauded CRISPR-Cas9 is DNA-guided... endonuclease NgAgo! We'll discuss how NgAgo is faring with users in a minute, but, to start, let's review...cleavage (1). Both the CRISPR-Cas9 and NgAgo genome engineering methods have their own pros and cons (2). The...protein to load an ssDNA guide. It is possible that, during translation, only a small fraction of nascent NgAgo...Development and applications of CRISPR-Cas9 for genome engineering." Cell 157.6 (2014): 1262-1278. PubMed PMID: ...

  5. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Introduction There are three primary methods for repairing DNA after a double-stranded break. HDR copies ...mutations seen with TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and...Non-homologous End Joining Read About Other Genome Engineering Tools like Sleeping Beauty Mutase Find the Ideal... genome engineering... Mary Gearing...

  6. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...special considerations. Conventional flow cytometry During conventional flow cytometry, light emitted by a...distinguish these two fluorophores from one another during spectral unmixing.     Figure 2: The ...a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...telling exactly how many Cy7 molecules are added during that particular batch. This can present particular...upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical to include...

  7. Transferable Skills: Negotiation

    Type
    Blog Post
    ...something done not by people wearing a lab coat, but instead by people wearing business suits discussing ...transferable skills while working in the lab and by offering advice on areas where you might need some help...you are negotiating. Then take a moment before, during, or after a negotiation to think about the situation...experience in the area I want to explore while also ensuring that my work gets completed? I want my company...

  8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...heterozygote into a homozygote and ensuring that all future offspring inherit the endonuclease gene. As...As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (...shared by everyone, he is deeply concerned with ensuring that gene drives and other collective technologies...selfish genes as tools for the control and genetic engineering of natural populations. Proc. Biol. Sci. 270,...

  9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...suppose you have a piece string that’s 10 meters long. You twist and roll the string into a tight ball. Now...in a way that brings linearly distant regions close together. gRNA Selection and Filtering for Off-target...Now the two ends of the string, instead of being 10 meters apart, are mere millimeters apart. Similarly...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    ...bacteria and prevents sensitive bacteria from acquiring resistance genes. Yosef et al. suggest that it...not previously infected by the lysogenic phage, rendering the population sensitive to antibiotics. Created... Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial...Addgene Blog CRISPR Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene ... Mary Gearing...
Showing: 401 - 410 of 1010 results