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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,019 results for: CAL;

Showing: 801 - 820 of 1019 results

  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...presence of the CPP may alter protein function or localization. Thus, the usefulness of CPPs must be evaluated...

  3. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring meetings a priority. Schedule meetings or calls regularly and don’t cancel, so that you drive progress...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...aqueous samples might be affected by dry ice, and specifically how dry ice can affect virus from Addgene. ...never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out. Conclusions...

  6. Components of CRISPR/Cas9

    Type
    Blog Post
    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...arrays, they did not know their functions and simply called the repeated elements “direct repeats” and the ...

  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability... and “Sequence” tabs. BLAST Use the Basic Local Alignment Search Tool (BLAST) via the NCBI website...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG...

  11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... a downregulation of signal, though it’s hard to call from this plot. The red (bottom) peak overlaps with...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

  14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... the following, which are later printed for my physical lab notebook, along with any figures generated...between each word can make it difficult to accurately call a file. Version control your commonly used scripts...

  15. CRISPR Library Amplification

    Type
    Protocol
    ...the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later...libraries up to 200,000 individual plasmids and can be scaled according to the actual number. Required Quality...selection). This recombination, at a low rate, is not typically a problem as these sequences are not efficiently...200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly. Pro-Tip...cells) Less library DNA can be used but this dramatically increases the chances of individual plasmids...Cell Spreaders (VWR, 60828-680) 5 mL and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and ...normal growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...out the sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably well...answer to these questions--which were real, not hypothetical--was clearly “no.” When there was room for misinterpretation...

  18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...once it is engaged by its target, it will non-specifically cut ssDNA. This feature is shared by other Cas... potential as a viral therapeutic that could specifically degrade target viral RNA. For more information...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...library, propagated it, separated it into roughly identical replicate groups, and treated these groups with...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

  20. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...Another strength of Golden Gate cloning is its scalability. Unique 4 base overhangs can be used to assemble...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...
Showing: 801 - 820 of 1019 results