We narrowed to 989 results for: IGH@;

...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

...Lambert-Eaton myasthenic syndrome (LEMS) because, at high concentrations (25 mM), Gdm+ binds to and inhibits...disease-linked Arg-mutant proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering...

...are a multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms ...AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population. These pre-existing...

...1948 as a variation of the more commonly observed light emission by fluorescence. The widespread use of ...distances that are too miniscule for conventional light microscopy. Don't FRET - Use these FRET resources...

...and BioID, any protein that’s within a ~10nm neighborhood of the bait protein will be biotinylated. This...Macneill, S., & Varnaitė, R. (2016). Meet the neighbors: Mapping local protein interactomes by proximity‐dependent...

...expression can change as well. The more frequently and tightly bound DNA is to histones, the more compacted it...occurs at CpG islands (regions of the DNA with a high frequency of C and G nucleotides). The conversion...

...of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose it can be. Let... Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with NEB’s DAD Interview...

... is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated to the...interest. The optical density (OD) - the measure of light absorbed by a solution - of these wells is plotted...

...for these RNA sequences (3 nM), however, was quite high and above what would be expected in patient samples...daunting. It took a true team effort and many late nights, not unlike most research, but the urgent need ...

...fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw cycles and should be stored...resistance marker expression following transfection, higher-level expression after approximately 72 hours is...

...gradually. I didn’t become Chair of a Committee overnight (however this can be done if you already have ...was involved with postdoctoral issues first at Brigham and Women’s Hospital/Harvard Medical School and...

...my experience the best set-up is panelists in a slight semi-circle (so they can see each other) on comfortable...redirect the conversation. The person you cut off might be miffed but the other 200 audience members will...

...think I need. Unlike lentivirus, AAV requires a higher number of viral particles for efficient gene transfer...only harvest the cell pellet. My yield would be higher if I used PEG precipitation, but for my experiments...

...gene knock-out (left) and targeted DNA knock-in (right) by CRISPR/Cas9. Adenoviral CRISPR vectors (left...left) or CRISPR vectors and donor DNA vectors (right) are transduced to target cells. Within target cells...

...cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab to simultaneously ... chromatin's dynamic role in gene regulation The high resolution and multiplexed chromatin ‘photos’ generated...

...bp, but pay attention to the sequence’s Tm, as a higher Tm give more efficient recombination. We normally... two colonies for simple cloning procedures. Use high fidelity DNA polymerase This will minimize random...