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Showing: 801 - 820 of 1076 results
  1. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...small circular pieces of DNA that replicate independently from the host's chromosomal DNA. They are mainly... markers for use in other cell types. Primer Binding Site A short single-stranded DNA sequence used ...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is...
  2. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ... and links provided, in addition to a list of individual screens curated for the publication. Clicking...blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library blog posts Check... guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your research ...
  3. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...(and, of course, find plasmids that will be useful to you.) With these goals in mind, we set out to update... plasmid class covered on that page. To help you find popular plasmids, we’ve also added four new plasmid...
  4. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...organization what do you think of? Maybe the term brings to mind a social service organization like the Red Cross...organization cannot distribute profits to private individuals through stock or investor pay outs. Addgene has...relationship with our community members. We also find that, as a nonprofit, we attract partnerships with...
  5. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...experience this very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments Running...protocols, guidelines, and standards. Bricking science reminded us of our inner children who have no preconceived...to Share Your Science Resources on Addgene.org Find Protocols and Protocol Videos Check out LeGO plasmids...
  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system...adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy the... CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have...
  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...
  8. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... path at a time, the variety of paths in life is mind-boggling. Unlike a mountain – with many routes up...stochasticity of molecular interactions and embedding individual diversity to get from gamete to a visibly complex... to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...
  9. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...understand the disease better but hopefully also to find a way to fix the problem. “We’ve had the opportunity...healthy cells into melanoma cells. They’ll combine induced pluripotent stem cell (iPS) technology and CRISPR-Cas9...Awardees for speaking with us! Additional Resources Find out more about the Rare Genomics Institute and the...
  10. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...business-focused?" The reason is that I knew that the mind-set of the employer was not such that he/she would...up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting,...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about...
  11. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...
  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...ends. This homology can be contained within the individual fragments themselves, but doesn’t necessarily... challenges can be circumvented by generating individual fragments containing mutations and aligned via...Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...
  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Articles with and without plasmids at Addgene To find out whether scientific articles associated with ...Addgene plasmids get more citations So what do we find? Lots more citations for the papers with plasmids...adjustment is done within publication years. What do we find once we do this adjustment? That papers with plasmids...
  14. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...have moderate to high immunogenicity, meaning they induce an immune response in the host. So why use a gamma-retrovirus...also has low immunogenicity, meaning it does not induce the same high immune response as retroviruses. ...immunogenicity, but some strains have been engineered to induce a lower inflammatory response within hosts. Relative...
  15. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...and/or opportunities) to modify the device for individual experiments. Manufacturers rarely provide the...for use, or they can modify and optimize the code, find and fix security-critical bugs, and contribute to...produced quickly with the printer, act here as a kind of "teleporter," since a part developed in Germany...
  16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...nuclease. This can be determined by measuring the indel frequency at a target site. A specificity ratio ...the Addgene blog Read our CRISPR 101 blog posts Find more information about Cas proteins Download the...plasmids and resources page Read our CRISPR guide Find CRISPR plasmids for mammalian systems ...
  17. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...strandedness) along with its action on the substrate (nick, bind, cleave, mixed). This information represents the...Considerations section on each enzyme page, you'll find a link to search Addgene's collection for plasmids...embedded in the References lists on every page you can find direct links to plasmids from the article if they're...
Showing: 801 - 820 of 1076 results