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We narrowed to 978 results for: LARS

Showing: 801 - 820 of 978 results

  1. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

  2. A Look at Addgene's QC Process

    Type
    Blog Post
    ...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...

  4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in a ready-to-use AAV5 viral...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...ease of use, droplet digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  15. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect method... drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    ... match their original description. The oldest, largest, and most diverse of these repositories is the ...out on low passage cells and this means you can regularly discard the cells you are working with because...

  18. Molecular Biology Reference

    Type
    Guide
    ...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Molecular Biology Basics...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing... molecule is known as DNA or molecular cloning . The process of molecular cloning enabled scientists to...Basics Origins of Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids...Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes as carriers of phenotypic...These findings culminated in the central dogma of molecular biology, that proteins are translated from RNA...chemistry enable isolation and purification of cellular components, such as DNA, but practically this ...

  19. Modular Cloning Guide

    Type
    Guide
    ... Molecular Biology Reference Molecular Cloning Techniques MoClo Guide Modular Cloning (MoClo...Plasmid Kits Blog: Plasmids 101 Modular Cloning Blog: Plasmids 101 Modular Cloning Applications and Kits...compatible with PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant...enabling larger multi-gene constructs, multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...Golden Gate Cloning Synthetic Biology Plasmids Modular Cloning (or MoClo) is a system for efficiently ...engineering, including CRISPR/Cas9 components. MoChlo: Modular Cloning Chloroplast Toolbox Plant Expression Scott...

  20. DNA Quantification

    Type
    Protocol
    ...Information During several different stages of molecular cloning, it is important to get a quick and accurate... a liquid to determine the concentration of a particular substance in that liquid. Molecules absorb different...
Showing: 801 - 820 of 978 results