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We narrowed to 1,035 results for: LAS

Showing: 801 - 820 of 1035 results

  3. Lab to Office Culture Shock

    Type
    Blog Post
    ...with our Transferable Skills Guide.     Over the last decade, my peers and I have tried to move away from...

  17. Chemogenetics Guide

    Type
    Guide
    ... J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals RF, Pomper... chemogenetics plasmids available through Addgene, check out our Chemogenetics Plasmid Collection . Figure...Preparations of Chemogenetic Plasmids ! Table 4. Common promoters in chemogenetics plasmids Promoter Cell Specificity...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity. Science...G-protein coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass ...different types of DREADDs that can be broadly classified by the signaling protein that the receptors couple...inhibit neuronal signaling by inhibiting adenylate cyclase and downstream cAMP production. There are currently...

  18. Optogenetics Guide

    Type
    Guide
    ...EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth... check out our Biosensors Plasmid Collection . To find optogenetics plasmids available through Addgene...mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids for your next experiment. For a ...domain (iLID plasmid), while its natural binding partner SspB is included in a separate plasmid; Blue light...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin... activated. Optogenetics tools can be broadly classified based on their functions into two groups: Actuators...Addgene, check out our Optogenetics Plasmid Collection . Microbial Opsins Opsins are light-gated ion channels...

  19. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...for verifying plasmids like this is "plasmid fingerprinting", where you cut the plasmid into 3-8 pieces...used to verify your plasmid. The goal of a diagnostic digest is to cut your plasmid into specific sized...insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting" Often, it will be enough...for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more. Protocols...Digest You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction...selectively move a specific piece of DNA from one plasmid to another. A diagnostic restriction enzyme digest...called restrictions sites. Often, the size of the plasmid insert and vector backbone are known and thus this...

  20. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture Background Information Plasmids can carry...copy regardless of plasmid size. See the plasmid's info page to determine if your plasmid is high or low ...resistance gene on a plasmids allows researchers to easily isolate bacteria containing that plasmid from bacteria... isolate your plasmid DNA from the bacterial culture by following Isolating Your Plasmid DNA . Antibiotic... low copy plasmids? The copy number refers to the number of copies of an individual plasmid within a single...number plasmids should only need to be grown for 12-16 hr on average. Certain features of a plasmid may ...clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture is capable of supporting...
Showing: 801 - 820 of 1035 results