We narrowed to 876 results for: NSI;

1. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...

2. Plasmids 101: Blue-white Screening

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   Blog Post

   ...making your plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving...

3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...

4. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ... activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they ...

5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...Lerner RA, Wigler M (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae...

6. RNA Extraction Without A Kit

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   ...samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of...

7. RaPID Detection of RNA-protein Interactions

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   ...Russell, P., & Guttman, M. (2014). Methods for comprehensive experimental identification of RNA-protein interactions...

8. dTAG - You're it!

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   ...or Jay Bradner ([email protected]). Considerations when getting the dTAG system up and running...

9. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...genes of interest. These transcription factors consist of the GAL4 or ZIF268 DNA binding domains fused...

10. Celebrating 15 Years of Scientific Sharing

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    ... new growth phase of the organization and the expansion of our impact, I’d like to take a moment and honor...

11. Summer SciComm: Preprints

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    ...funding bodies and review committees encourage and consider preprints as demonstration of productivity. Part...

12. Summer SciComm Series: Cognitive Load

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    ...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a...

13. Transferable Skills Guide: Problem Solving

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    ...did not just automatically come to a solution. Consider the thoughts that went through your head as you...

14. Viral Vectors 101: An Introduction to AAV

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    ...and in vivo laboratory experiments ranging from transient gene expression in a particular cell type to CRISPR...

15. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...completely normal to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I'...

16. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...oxygen. Other useful backbone modifications to consider include amide linkages, unlocked nucleic acids...

17. CRISPRainbow and Genome Visualization

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    ...exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene copy number based on the...

18. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...salary information as of December 1st. Salary inconsistencies across geographical regions When we originally...

19. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...complementarity to the gRNA, they have a higher propensity to reform double helices and decrease Cas9 cutting...

20. The Future of Research Symposium Boston 2015

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    ... to train our young scientists for futures both inside and outside of academic research (4). There are...