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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,043 results for: STI;

Showing: 801 - 820 of 1043 results

  1. Let There Be LITE Plasmids

    Type
    Blog Post
    ...anchors for genome engineering applications was still very new. Now, Konermann and her colleagues including...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...

  2. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

  3. Reflections and Looking Ahead

    Type
    Blog Post
    ...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...repository can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility...

  4. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

  8. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure...

  9. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...

  10. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ..., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may...

  12. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents...

  13. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...

  14. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...

  19. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...
Showing: 801 - 820 of 1043 results