We narrowed to 1,045 results for: STI;

...individual plasmids to 5,000 different research institutions. This has given us a unique window into technology...plasmid distributed in our 1st year, 2004, and it still gets requested! Fire Lab C. Elegans Kit - Our first...

...anchors for genome engineering applications was still very new. Now, Konermann and her colleagues including...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...repository can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility...

...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

...the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...at a more-typical scale of tissue culture while still offering the potential for true biological insight...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...

...CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single genes in immune...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases...

... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...

..., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may...

... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents...

...frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...

...localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...

...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally... than through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate...

...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

...recombination at pseudo- or cryptic loxP sites (estimated to occur at a frequency of 1.2 per megabase in...toxicity of this enzyme in Drosophila. If you're still reading this post and you don't work in Drosophila...

...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...categories: Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red,...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

... but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is ...appears between each spacer in the CRISPR array, distinguishing the foreign sequences and keeping the array...