We narrowed to 934 results for: TIM;

1. The Importance of High Titer for AAV Transductions

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   ...Nonetheless, there are a variety of ways you can optimize AAV production. We’ve listed some simple steps...

2. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   ...more specific procedural instructions, including timing, preparing the smear, and mixing the stains and...

3. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   ...scientists are using sgRNA for selection and optimization. In addition, the current work in developing...

4. Viral Vectors 101: Inverted Terminal Repeats

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   ...not properly assemble, but these sequences can sometimes be removed to improve vector stability and sequencing...

5. Hot Plasmids - January 2023

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   ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...

6. Four Factors that Differentiate the Stem Cell Field

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   ...deeper into the world of stem cell scientists. In my time at the meeting, I've made some observations regarding...

7. R Bodies: Membrane-Rupturing Microscopic Tools

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   ...then amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical...

8. Resume Writing for Non-academic Science Careers

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   Blog Post

   ...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...

9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...however, that there is no single fluorescent protein optimal for all organisms and that if you want the brightest...

10. Plasmids 101: Mammalian Vectors

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    Blog Post

    ...the foreign DNA will be degraded or diluted over time. This, however, may not be a problem depending on...

11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ... may be undesirable in many applications. At the time of our first blog post on choosing a Cas9, the Feng...

12. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...

13. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...interactions between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF...

14. Improved Plasmid Maps Powered by SnapGene

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    Blog Post

    ...will cut (in basepairs), and the total number of times they cut. Use the filters on the right to find single...

15. Addgene’s Viral Service - Why Virus? Why Now?

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    ...directions. I would like to thank them for their time, dedication, advice and encouragement. Why virus...

16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...harness the natural diversity of CRISPR systems, only time will tell how large the genome and transcriptome...

17. Hot Plasmids - May 2022

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    ... And, while the BLITZ system was designed and optimized with zebrafish in mind, it can easily be applied...

18. Antibodies 101: Isotypes

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    Blog Post

    ...encounters  an antigen for a second or subsequent time. It is expressed as a monomer with a valency of ...

19. Viral Vectors 101: Viral Vector Elements

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    ...used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and...

20. Viral Vectors 101: Types of viruses

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    ...lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends ...