We narrowed to 936 results for: TIM;

1. FPbase: A new community-editable fluorescent protein database

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   Blog Post

   ...these tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current ...

2. Pushing the Limits of DNA Assembly

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   ...transformation can take 5 days. NEBuilder shortens that time to one day by simplifying the protocol to a single...

3. New and Upcoming Viral Vectors - June 2019

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   ...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

4. The Importance of High Titer for AAV Transductions

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   ...Nonetheless, there are a variety of ways you can optimize AAV production. We’ve listed some simple steps...

5. Improving Transduction Efficiency with Exosome AAVs

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   ...PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...

6. Plasmids 101: Gram Negative and Positive Bacteria

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   ...more specific procedural instructions, including timing, preparing the smear, and mixing the stains and...

7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   ...scientists are using sgRNA for selection and optimization. In addition, the current work in developing...

8. Four Factors that Differentiate the Stem Cell Field

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   ...deeper into the world of stem cell scientists. In my time at the meeting, I've made some observations regarding...

9. R Bodies: Membrane-Rupturing Microscopic Tools

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   ...then amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical...

10. Resume Writing for Non-academic Science Careers

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    Blog Post

    ...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...

11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...however, that there is no single fluorescent protein optimal for all organisms and that if you want the brightest...

12. Plasmids 101: Mammalian Vectors

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    ...the foreign DNA will be degraded or diluted over time. This, however, may not be a problem depending on...

13. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...

14. Viral Vectors 101: Viral Vector Elements

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    ...used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and...

15. Viral Vectors 101: Types of viruses

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    ...lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends ...

16. Viral Vectors 101: Viral Applications

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    ...simultaneously in your system instead of just one at a time. Many of these libraries are packaged and delivered...

17. Viral Vectors 101: Viruses as Biological Tools

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    ...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

18. How Addgene Creates Content

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    Blog Post

    ...in the areas in which they have expertise. Other times, we may need to reach out to an external expert ...

19. Antibodies 101: Isotypes

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    Blog Post

    ...encounters  an antigen for a second or subsequent time. It is expressed as a monomer with a valency of ...

20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...harness the natural diversity of CRISPR systems, only time will tell how large the genome and transcriptome...