We narrowed to 934 results for: TIM;

1. Hot Plasmids - January 2023

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   ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...

2. Plasmids 101: Gram Negative and Positive Bacteria

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   ...more specific procedural instructions, including timing, preparing the smear, and mixing the stains and...

3. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   ...scientists are using sgRNA for selection and optimization. In addition, the current work in developing...

4. Viral Vectors 101: Inverted Terminal Repeats

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   ...not properly assemble, but these sequences can sometimes be removed to improve vector stability and sequencing...

5. Pushing the Limits of DNA Assembly

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   ...transformation can take 5 days. NEBuilder shortens that time to one day by simplifying the protocol to a single...

6. Four Factors that Differentiate the Stem Cell Field

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   ...deeper into the world of stem cell scientists. In my time at the meeting, I've made some observations regarding...

7. R Bodies: Membrane-Rupturing Microscopic Tools

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   ...then amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical...

8. Resume Writing for Non-academic Science Careers

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   ...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...

9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   ...however, that there is no single fluorescent protein optimal for all organisms and that if you want the brightest...

10. Plasmids 101: Mammalian Vectors

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    ...the foreign DNA will be degraded or diluted over time. This, however, may not be a problem depending on...

11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...

12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...harness the natural diversity of CRISPR systems, only time will tell how large the genome and transcriptome...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ... may be undesirable in many applications. At the time of our first blog post on choosing a Cas9, the Feng...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...

15. Viral Vectors 101: Types of viruses

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    ...lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends ...

16. Viral Vectors 101: Viral Applications

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    ...simultaneously in your system instead of just one at a time. Many of these libraries are packaged and delivered...

17. Viral Vectors 101: Viruses as Biological Tools

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    ...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

18. How Addgene Creates Content

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    ...in the areas in which they have expertise. Other times, we may need to reach out to an external expert ...

19. Hot Plasmids - May 2022

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    ... And, while the BLITZ system was designed and optimized with zebrafish in mind, it can easily be applied...

20. Antibodies 101: Isotypes

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    ...encounters  an antigen for a second or subsequent time. It is expressed as a monomer with a valency of ...