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...techniques that you can use antibodies for in our Intro to Antibodies post Resources on Addgene.org Browse...

...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could...

...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

... in the hands of the Doench lab. Additionally, controls available in the system are validated and were...

...boiling point of water. Dr. Terns gave a great introduction to the repeated and distinct aspects of the ...

...living cells where methylation of these sites was controlled, the reporter consistently showed significantly...

...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...

...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...