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Showing: 801 - 820 of 1060 results
  1. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...dice on a new gRNA in the future! References and Resources References Eva K. Brinkman, Tao Chen, Mario ... frame editing frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, ...
  2. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post
    ...recent research @daria_gift_shch.     References and resources References Shemetov, A.A., Oliinyk, O.S. & ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...
  3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires a PAM...femto-aptomolar range. For more details on the differences between these methods, check out this table.  ...can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari, A. H., Sinha,...
  4. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...Gram-negative bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa...for allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...
  5. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ...on unnecessary materials, or even a loss of the offer all together. The problem is that the discussions...specialized equipment, try finding institutes that offer shared equipment that you can use at a lower cost...turned down a faculty position because none of the offering institutes were able to provide the resources ...
  6. Hot Plasmids: Spring 2025

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    Blog Post
    ...control of one of seven enhancers specific for different interneuron populations. The enhancers span all...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...effort to develop molecular tools for studying different brain cell types. Look for more of these interneuron-targeting...
  7. Sequencing Options for CRISPR Genotyping

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    Blog Post
    ...irrespective of in silico prediction. These techniques differ in important ways, but can also complement one ...sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer to the clinic...https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target...
  8. It’s CRISPR Week at Addgene!

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    Blog Post
    ...Twitter feed will be all about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start... Jennifer Tsang...
  9. New FLIS Plasmid (April Fools 2022)

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    Blog Post
    ... this new technology as helpful as we have!  References and Resources Additional resources on the Addgene...Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...
  10. We’re Going Viral This Week!

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    Blog Post
    ...viral vector information. Each day, we’ll cover a different topic as shown in the graphic below. Follow the... Jennifer Tsang...
  11. Twenty Years of Sharing Science!

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    Blog Post
    ... to 764 scientists at 375 organizations in 21 different countries. It's safe to say we keep our shipping...science support to our efficient and easy tech transfer process to our website and educational resources...
  12. SciComm with the Experts at Science in the News Part 1

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    Blog Post
    ... the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists. These programs ...Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks...
  13. Bringing Renewable Energy to Addgene

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    Blog Post
    ...higher percentage of renewable sources. This program offers options of between 14% - 100% electricity supplied...have chosen to participate at the highest level offered. As a result, we will be drawing 100% of our electricity...
  14. CRISPR Cheat Sheet

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    Blog Post
    ... May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp new CRISPR material ...and destroy viruses that infect them. Commonly referred to as a bacterial immune system. Genome Editing...
Showing: 801 - 820 of 1060 results