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We narrowed to 971 results for: lars

Showing: 801 - 820 of 971 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in a ready-to-use AAV5 viral...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...ease of use, droplet digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa...

  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect method... drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...

  11. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    ... match their original description. The oldest, largest, and most diverse of these repositories is the ...out on low passage cells and this means you can regularly discard the cells you are working with because...

  14. DNA Quantification

    Type
    Protocol
    ...Information During several different stages of molecular cloning, it is important to get a quick and accurate... a liquid to determine the concentration of a particular substance in that liquid. Molecules absorb different...

  15. Protocol - How to Design Primers

    Type
    Protocol
    ...also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  16. A Control for All Seasons

    Type
    Blog Post
    ...both groups on a western blot and look for the molecular weight shifts indicative of tau phosphorylation...against a protein standard (P) to check for tau molecular weight shifts. A negative control is a sample...

  17. Protocol - How to Streak a Plate

    Type
    Protocol
    ... plate, do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ... looking at a process-oriented team serving a particular function. Project team A Project Team is assembled...an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  But some...

  20. Ligation Independent Cloning

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...Transform Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng ...
Showing: 801 - 820 of 971 results