...which refers to mEGFP. This variant was made by introducing two mutations (F64L, S65T) in the original jellyfish...fluorescent proteins can be accurately determined in vitro by measuring the fluorescence intensity of purified...Peptide-tags for site-specific protein labelling in vitro and in vivo." Molecular BioSystems 12.6 (2016): ... "Blue fluorescent amino acid for biological spectroscopy and microscopy." Proceedings of the National...Additional Resources on the Addgene Blog Read our Introduction to GFP Read our Fluorescent Proteins Blog Posts...

...for with in vitro display methods such as phage display (Bradbury et al., 2011). In vitro selection avoids...expressed as scFvs and screened with in vitro assays to select for strong binders. Then the sequences for the...2011) Beyond natural antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 ...

...least 3 months experience working in a developing tropical country. This is why I set up a placement working...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when...very new, and everything was more structured and controlled. I worked from Standard Operating Procedures ... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...

...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...expresses the VSV-G envelope protein under the control of the powerful mammalian CMV promoter. Envelope...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ... Guide to All Things Lentivirus 5 Tips for Troubleshooting Viral Transductions Getting the Most from Your...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

...offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL...necessary MTA, then start production and quality control.  Our packaged on request AAV service uses the ...the same trusted production and quality control processes as our in-stock service: ddPCR, purity testing...blog post on viral vector production and quality control, our AAV Packaged on Request webpage, or our announcement...

... AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng ...from the Gradinaru lab. This is useful for both in vitro and in vivo work with many cell types, as CAG is...serotypes pAAV-hSyn-GRAB_OTmut AAV9 Biosensors (Control) Li New viral service pAAV-hSyn-GRAB_ACh3.0 AAV9...

...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine...modified cells; and (5) is compatible with the introduction of unique molecular identifiers, which can improve...includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen, Ovalbumin, into cell lines...

...m an introvert!” is not an excuse. Every psych test I’ve ever taken has declared me an introvert, and ...Scientist who coordinates all kit creation and quality control efforts with depositors; Gizela, the lab member...deposits into the repository, performing quality control, and providing technical customer service. Team...easier. Trust Ultimately, your team could have a strong sense of purpose, an excellent leader, and perfectly...

...Transfection grade DNA Figure 1: Transformation introduces transformation grade plasmids into a bacterial...bacterial transformation of chemically and electrocompetent cells is minimal (picogram range). It can ...2.2, and provide an indication of purity versus chaotropic agents as guanidine thiocyanate and guanidine...Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian cells? Use transfection...