We narrowed to 872 results for: cel

...fluorescent proteins available. Fortunately, several excellent reviews have collated data from various papers... monitoring sensitized emission in living plant cells." (2013). PubMed PMID: 24194740. PubMed Central ... alters the FRET efficiency. Detecting certain cellular changes with an intermolecular probe is often ...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time...of labs to answer sophisticated questions about cellular processes. Currently, FRET experiments can probe...concentration or activity of small molecules, detect cellular processes and signaling cascades, quantify mechanical...-molecule FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to...

...measuring protein expression or localization in cells, or even single-particle tracking of the bound complex...) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent protein tags. ...(1–10) and GFP(11) Versatile protein tagging in cells with split fluorescent protein. Kamiyama et al. ...pairs with sfCherry2(11) Multiplexed labeling of cellular proteins with split fluorescent protein tags. ...tracking of protein-protein interactions in living cells. Shao et al. Commun Biol. 2021 Yujie Sun   For...bright enough for single-molecule tracking in live cells.  Controls  When designing experiments, it’s important...

...its oxidized derivatives. Cell 152, 5 P1146-1159. https://doi.org/10.1016/j.cell.2013.02.004.  Xu X, Tao...targeted DNA demethylation. Cell Discov 2, 16009. https://doi.org/10.1038/celldisc.2016.9.   ...enzymatic activity cause demethylation?  Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy...methylate sites blocked by dCas9. Within a few rounds of cell division, the original methylated strand is diluted...dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing these constructs can...can be achieved by fluorescence-activated cell sorting, and stable gRNA expression can be achieved using...TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated somatic enhancers. Nat...

...Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to determine...DNA and how many cells to use for your transformation(s). The amount of competent cells you need total ... generally electroporated into electrocompetent cells to ensure the highest possible transformation efficiency...efficiency. Using Stbl4 or other ultra-high efficiency cells ensures that plasmid diversity in the library is...transformation may vary depending on the particular type of cells you are using, as well as other factors, such as...transformations, such as splitting 400 ng of DNA in 100 μL of cells over four individual electroporations. The specifics...do see recombination, we recommend ensuring the cells used are meant for repetitive sequences (such as...

...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor...antigenic drift.    Starr et al Cell. 2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science...Library generation and sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations with... SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used the library to identify... candidates were validated by IP-MS using HEK293 cell lysate where the antigens would be in their native...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue their ...protect proteins from unfavorable interactions in the cell, improving biosynthetic pathway yields by stabilizing...

...metabolite in a cell. The only way to do that at scale right now is to grow up a bunch of cells, sacrifice ...capable of storing data and computing inside living cells. He's also a co-founder of the BioBricks Foundation...transistor-like digital genetic switches that enable cellular computing. These logic gates built from transcriptors...as an operating system for engineering an entire cell. Typically when people use plasmids, they are trying... you could imagine engineering the entirety of a cell. That's a big long-term technical challenge. We ...That's central to everything that goes on inside a cell. Until we get a handle on precise, forward engineering...scale, it's going to be tricky to tame the rest of a cell. The BIOFAB plasmids represent the first systematic...

...used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG tag...studies (Fujita et al., 2018). In addition to the "in cell" form of this method, in which an engineered DNA-binding...molecule such as the CRISPR complex is expressed in cells to be analyzed, the Fujii lab also developed another...recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed PMID: 26848818. Fujita...between genomic regions by enCh IP‐Seq." Genes to Cells22.6 (2017): 506-520. PubMed PMID: 28474362. Fujita...‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 (2018): 318-325. PubMed PMID: 29480524. Gilbert...RNA-guided regulation of transcription in eukaryotes." Cell 154.2 (2013): 442-451. PubMed PMID: 23849981. PubMed...

...reach essentially every cell or else small pools of antibiotic resistant cells could repopulate the environment...strategies are nonspecific - they harm any bacterial cell without a resistance gene, allowing resistant bacteria...antibiotics target one of three essential processes: cell wall synthesis, DNA replication, or protein synthesis...eliminated from the bacterium, which may also induce cell death. CRISPR also enables increased specificity...Treating a mixed population, represented here by cells of different colors, specifically targets bacteria...bacteria. Targeting a genomic sequence results in cell death. Plasmid targeting may result in plasmid loss...and antibiotic resensitization, or may also cause cell death. Image from Beisel et al., licensed under ...

...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage)....extensively to screen for molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA...field of eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully...The reporter gene then allows detection of those cells where binding between the proteins of interest has...alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If the problem is that your proteins are not post-translationally... DNA and activate a reporter gene in eukaryotic cells, fusion proteins must gain access to the nucleus... the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729-736. PubMed PMID: 3907859. 4. Fields...

...Xenopus frog cell lines to develop robust methods for frog gene editing. They edit in a cell line, then ...is the smallest photosynthetic cell in existence, but most abundant cell on earth (twice the human biomass...only 1,700 genes total). Hydra (Celina Juliano) This is a known stem cell and regeneration model. Little...Mormyridae (Jason Gallant) Weakly electric fish are excellent systems to study for convergent evolution because...Hawaiian bobtail squid has an extreme number of nerve cells and an unusually high level of RNA recoding in the...using novel vesicles to efficiently transform these cells which don’t like foreign DNA at all (maybe because...