...Gel Procedure Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...the denaturing. Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set...protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do if my... to work efficiently. Taq Buffer with MgCl 2 : Provides an optimal and stable chemical environment for...