We narrowed to 916 results for: IRE;

1. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   ...proteins. Affinity purification methods typically require a protein to be tagged, which limits these approaches...

2. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

3. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...results we hoped for, but it did open up a new direction for the project. I was invited to participate ...

4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ...plasmids, glycoproteins and other proteins that require post-translational modification may not be easily...

5. 7 Tips to Secure a STEAM Internship This Summer

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   Blog Post

   ...Furthermore, companies across most industries tend to hire their former interns to fill entry-level positions...

6. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ...are many ways a user can input their query — by directly typing terms in a search bar, by ticking some ...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

8. How to Make Friends and Meet People at a Scientific Conference

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   Blog Post

   ...the person you are talking to is really in your direct area.  Review the conference schedule and attendance...

9. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   ...is helpful to find peers that share a similar direction or problem. For example, a group of people who...

10. dTAG - You're it!

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    Blog Post

    ...Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in...

11. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...endothelial cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal...

13. Antibodies 101: Conjugation

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    Blog Post

    ... 2012). And, you can also conjugate antibodies directly to beads for purification purposes.  Coupling...

14. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...chemical modifications to the RNA to control the entire editing system with a simple LED light. Lighting...

15. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...collaboration and growth opportunities, and gives your entire audience a chance to comfortably engage with you...

16. Addgene: The First Twenty Years

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    Blog Post

    ...to be young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's...

17. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...not so simple Just as our lab used jargon that required context to understand, I came to realize that ...

18. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...it would take years to develop the mouse lines required for this purpose. Tang wondered if he could use...

19. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...Scientific Manuscripts. Any scientific manuscript will require numerous other references to scientific literature...

20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    ...GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing of...