Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 979 results for: LARS

Showing: 821 - 840 of 979 results

  1. DNA Quantification

    Type
    Protocol
    ...Information During several different stages of molecular cloning, it is important to get a quick and accurate... a liquid to determine the concentration of a particular substance in that liquid. Molecules absorb different...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ...also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  3. A Control for All Seasons

    Type
    Blog Post
    ...both groups on a western blot and look for the molecular weight shifts indicative of tau phosphorylation...against a protein standard (P) to check for tau molecular weight shifts. A negative control is a sample...

  4. Protocol - How to Streak a Plate

    Type
    Protocol
    ... plate, do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ... looking at a process-oriented team serving a particular function. Project team A Project Team is assembled...an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  But some...

  7. Ligation Independent Cloning

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...Transform Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng ...

  8. Video Library

    Type
    Protocol
    ...success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key...samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based...

  10. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly purified and... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

  12. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...from academic viral vector cores. Our titers were similar to those reported by these institutions. This protocol...Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular Weight: 6208 bp x 650 dalton/bp or (g/mole)/bp...

  13. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified recombinant...

  14. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...empirically, and that the cell population be monitored regularly for transgene expression. This protocol has been...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...37°C, 5% CO 2 overnight. Although cells should regularly be passaged in DMEM + 10% FBS with penicillin/...limited to the implied warranties of fitness for a particular purpose, non-infringement, typicality, safety...

  16. CRISPR Guide Update

    Type
    Blog Post
    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

  17. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

  18. The 12 Days of CRISPR

    Type
    Blog Post
    ...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...

  20. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...community of creative scientists delivered some spectacular photos once again this year! All winners receive...
Showing: 821 - 840 of 979 results