We narrowed to 876 results for: NSI;

1. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   ...assembling the toolbox needed for anyone to begin considering genetic engineering projects in a very big way...

2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ... starting point for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally...

3. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...Arg-mutant proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from ...

4. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...functions of key regeneration-related genes in four inexpensive and commercially available planarians: Girardia...

5. Hot Plasmids - October 2020

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   ...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity to be ...

7. Science communication: 9 Strategies to Get Your Foot in the Door

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   Blog Post

   ...full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The...

8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   Blog Post

   ... a number of different options. By carefully considering your ncRNA of interest, you should be able to...

9. Plasmids 101: Modular Cloning

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   ...Scripps Research, where he works on genetic code expansion.  References and Resources References Weber E...

10. CRISPR in the Clinic

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    Blog Post

    ...Off-target effects are also important safety considerations for all CRISPR therapies, but especially concerning...

11. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang Ligation Fidelity...

12. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...cells are behaving normally can be prohibitively expensive for many labs. We’ve created and tested plasmids...

13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...us at [email protected]. These deposits are considered large deposits and are exceptions to our standard...

14. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post

    ...of toehold switch-based RNA sensors within an extensive infrastructure of molecular and electronic companion...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...

16. Plasmids 101: FLEx Vectors

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    ...inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts...

17. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...it’s too inefficient and the reagents are too expensive to make it practical - but we have members looking...

18. Transferable Skills Guide: Managing a Team

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    Blog Post

    .... They will make mistakes and you will take responsibility (within reason!).  Try to buffer them from ...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final...

20. Transferable Skills: Negotiation

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    ... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...