We narrowed to 1,020 results for: NTS;
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TypeBlog Post...understanding of a GPCR mediated response. Acknowledgements Special thanks to Jeff DiBerto, a graduate...fluorescent protein plasmids Find biosensors for your experiments ...
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Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post...guidance of one of the faculty members, I helped grad students who were teaching for the first time. I walk[ed...called “Teaching Your Research.” This course has participants take their research from the bench to the classroom... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post... over 11,000 conference registrants, including scientists from six continents who dropped by the booth... -
Choosing Your Perfect Empty Backbone
TypeBlog Post.... To help you find the vector that fits your experiments, I've described below some of the most frequently...backbone. Or just ask – leave a message in the comments below and an Addgenie will help you find what ... -
Choosing a Good Mentor for Scientists
TypeBlog Post...scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose... than once to get a full picture and listen for hints at the same concerns from multiple people. Listen... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into... -
Viral Vectors 101: Transductions
TypeBlog Post...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...jGCaMP7 (keep reading) and will be releasing more variants in the next few months (see “coming soon”). The... can also be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool.... -
Summer SciComm: Science Storytelling
TypeBlog Post...communicators also use the hero’s journey, stories from patients or other affected people, man/animal vs nature... the Addgene blog SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series ... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...knowledge base to describe aptamers and SELEX experiments. Database, bas006 (2012). PubMed. For a stepwise... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...application is clinical; can we find ways to treat patients? Over a quarter of a million people have some ...activate a set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness... -
K. phaffii: Rising to the Occasion
TypeBlog Post...feed. Due to increasing prices of methanol and advancements in other biomass production techniques in the...suited to it. Putting K. phaffii to work The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...impact on the samples. We therefore devised a few experiments to determine if our tubes were permeable to the... the sample, which may have some effect on the contents of the sample. For example, one study found that... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...for pilot experiments and for interpreting the results of studies. When planning AAV experiments, scientists... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...temporal arrangement of chromosomes and how these arrangements may affect nuclear processes. The latest of ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...