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We narrowed to 1,010 results for: PLE

Showing: 821 - 840 of 1010 results

  1. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...Got You Here Won't Get You There: How Successful People Become Even More Successful, by Marshall Goldsmith...

  2. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...e.g. pLKO.1 - TRC cloning vector). Check out our complete guide to empty backbones for additional details...

  4. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...(on a schedule) I hope you all know by now that people get jobs at least 85% of the time because of relationships...pretty easy to make 30 minute appointments with people all over the world. Think about contacting alumni...

  6. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...

  8. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...PCR product. If you need to decrease error rate, please use one of these methods to ensure your insert ...

  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...

  10. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  12. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...and transgene expression (Zhou et al. 2017). For example, mutations in the RBE site reduce binding with ...AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different...

  13. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...representation is most easily understood visually. As an example, the curves from our recent amplification of the...

  14. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...change to a slide, think about whether the same principle applies to other slides in your deck.  If someone...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene...double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control...

  18. Antibodies 101: Isotypes

    Type
    Blog Post
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

  20. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...inspires students and teachers to apply engineering principles to biological systems. Today, what started in... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub...
Showing: 821 - 840 of 1010 results