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  3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...

  4. New CRISPR Web Resources and #12DaysofCRISPR Recap

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    Blog Post
    ...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...

  6. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

  7. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...

  8. SunTag and Fluorescent Imaging

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    Blog Post
    ...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...

  9. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ...

  11. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

  12. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...

  13. Advice for Choosing a Research Project

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    Blog Post
    ...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

  14. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post
    ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

  15. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

  16. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...

  17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...

  19. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...
Showing: 821 - 840 of 1011 results