We narrowed to 1,045 results for: STI;

...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...

... we can predict the resulting band sizes when digesting the plasmid sequence with one or more enzymes....Reference Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ...

... but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is ...appears between each spacer in the CRISPR array, distinguishing the foreign sequences and keeping the array...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

...analysis, Tm and primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly...

...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ...

...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is... Addgene is that we cover all of the shipping logistics and facilitate all material transfer agreements...

... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ...

...CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized...

... I've been answering biology customer service questions for nearly 7 years, and my colleagues and I have... worked so hard to make this poster, offered suggestions and tips, and helped get me to this meeting. ...

...plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding...cell division (bacteriostatic); however, the distinction between the two categories may be a bit of a ...

...function is inactivated by the separation of its synergistic domains. This paper demonstrated that the migration...mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance...

... show that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that...you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that! How...

...Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine...Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling...

...stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring. ... Christina Mork...

...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...

...of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...

...world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we began to receive urgent...Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand...

...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-time. Find plasmids...