We narrowed to 924 results for: TRO

1. Hot Plasmids: Winter 2025

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   Blog Post

   ...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

2. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

3. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

4. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

5. 10 Basic tips for mammalian cell culture

   Type

   Blog Post

   ..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

7. Transferable Skills: Negotiation

   Type

   Blog Post

   ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

8. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

9. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

10. Transferable Skills Guide: Managing a Team

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    Blog Post

    ...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

11. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...

12. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

13. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

14. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

15. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...  Susanna Stroik...

16. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

17. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

18. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

19. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post

    ... vectors that can be administered systemically (retro-orbitally, RO; or intracerebroventricularly, ICV...

20. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...